Rabbit PLK2 Polyclonal Antibody | anti-PLK2 antibody

PLK2, CT (Serine/Threonine-protein Kinase PLK2, Polo-like Kinase 2, PLK-2, Serine/Threonine-protein Kinase SNK, Serum-inducible Kinase, SNK) (PE)

Cat. Num. AAA6493998

• Gene Names: PLK2; SNK; hSNK; hPlk2
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PLK2; Polyclonal Antibody; CT (Serine/Threonine-protein Kinase PLK2; Polo-like Kinase 2; PLK-2; Serine/Threonine-protein Kinase SNK; Serum-inducible Kinase; SNK) (PE); anti-PLK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SNK. Species Crossreactivity: mouse, rat.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PLK2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide corresponding to a fragment within amino acids 375-406 in the C-terminal region of human SNK.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SNK in PMA-treated Pam212 cell lysate (lane 1) and rat testis tissue lysate (lane 2) usingMBS628060.)

Immunohistochemistry (IHC)

(IHC staining of formalin-fixed and paraffin-embedded human breast carcinoma tissue usingMBS628060. Peroxidase conjugated secondary antibody and DAB staining were used for visualization. )

Product Categories/Family for anti-PLK2 antibody

Antibodies; Protein Kinases

References

1. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002). 2. Liby, K., et al., DNA Seq. 11:527-533 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 93004081
• NCBI GeneID: 10769
• NCBI Accession #: NP_006613
• NCBI GenBank Nucleotide #: NM_006622
• UniProt Accession #: Q9NYY3
• Molecular Weight: 78kDa
• NCBI Official Full Name: serine/threonine-protein kinase PLK2 isoform 1
• NCBI Official Synonym Full Names: polo like kinase 2
• NCBI Official Symbol: PLK2
• NCBI Official Synonym Symbols: SNK; hSNK; hPlk2
• NCBI Protein Information: serine/threonine-protein kinase PLK2
• UniProt Protein Name: Serine/threonine-protein kinase PLK2
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PLK2
• UniProt Synonym Gene Names: SNK; PLK-2; hPlk2; hSNK
• UniProt Entry Name: PLK2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the polo family of serine/threonine protein kinases that have a role in normal cell division. This gene is most abundantly expressed in testis, spleen and fetal tissues, and its expression is inducible by serum, suggesting that it may also play an important role in cells undergoing rapid cell division. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

PLK2: a kinase of the PLK family. Participates in a mitotic checkpoint in which p53-dependent activation Plk2 prevents mitotic catastrophe following spindle damage. Plk2(-/-) embryos show retarded growth and skeletal development late in gestation. Apparently plays a role for Plk2 in the cell cycle.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Tumor suppressor; EC 2.7.11.21; Protein kinase, Other; Other group; PLK family

Chromosomal Location of Human Ortholog: 5q12.1-q13.2

Cellular Component: centriole; centrosome; dendrite; intracellular

Molecular Function: protein serine/threonine kinase activity; protein binding; signal transducer activity; protein complex binding; ATP binding

Biological Process: regulation of centriole replication; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of protein binding; mitotic cell cycle checkpoint; regulation of synaptic plasticity; protein amino acid phosphorylation; memory; mitotic spindle organization and biogenesis; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; Ras protein signal transduction; Rap protein signal transduction; G1/S transition of mitotic cell cycle; negative regulation of apoptosis