Rabbit PLK1 Polyclonal Antibody | anti-PLK1 antibody

PLK1 Antibody

Cat. Num. AAA9405929

• Gene Names: PLK1; PLK; STPK13
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: PLK1; Polyclonal Antibody; PLK1 Antibody; PLK; STPK13; anti-PLK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total PLK1 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 603

• Applicable Applications for anti-PLK1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:200; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human PLK1.
• Target Name: PLK1
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of HeLa cell lines, using PLK1 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cell using PLK1 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-PLK1 antibody

At least four distinct polo-like kinases exist in mammalian cells: PLK1, PLK2, PLK3, and PLK4/SAK (1). PLK1 apparently plays many roles during mitosis, particularly in regulating mitotic entry and exit. The mitosis promoting factor (MPF), cdc2/cyclin B1, is activated by dephosphorylation of cdc2 (Thr14/Tyr15) by cdc25C. PLK1 phosphorylates cdc25C at Ser198 and cyclin B1 at Ser133 causing translocation of these proteins from the cytoplasm to the nucleus (2-5). PLK1 phosphorylation of Myt1 at Ser426 and Thr495 has been proposed to inactivate Myt1, one of the kinases known to phosphorylate cdc2 at Thr14/Tyr15 (6). Polo-like kinases also phosphorylate the cohesin subunit SCC1, causing cohesin displacement from chromosome arms that allow for proper cohesin localization to centromeres (7). Mitotic exit requires activation of the anaphase promoting complex (APC) (8), a ubiquitin ligase responsible for removal of cohesin at centromeres, and degradation of securin, cyclin A, cyclin B1, Aurora A, and cdc20 (9). PLK1 phosphorylation of the APC subunits Apc1, cdc16, and cdc27 has been demonstrated in vitro and has been proposed as a mechanism by which mitotic exit is regulated (10,11).Substitution of Thr210 with Asp has been reported to elevate PLK1 kinase activity and delay/arrest cells in mitosis, while a Ser137Asp substitution leads to S-phase arrest (12). In addition, while DNA damage has been found to inhibit PLK1 kinase activity, the Thr210Asp mutant is resistant to this inhibition (13). PLK1 has been reported to be phosphorylated in vivo at Ser137 and Thr210 in mitosis; DNA damage prevents phosphorylation at these sites (14).

Product Categories/Family for anti-PLK1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 21359873
• NCBI GeneID: 5347
• NCBI Accession #: NP_005021.2
• NCBI GenBank Nucleotide #: NM_005030.5
• UniProt Accession #: P53350; Q15153; Q99746
• Molecular Weight: 68,255 Da
• NCBI Official Full Name: serine/threonine-protein kinase PLK1
• NCBI Official Synonym Full Names: polo-like kinase 1
• NCBI Official Symbol: PLK1
• NCBI Official Synonym Symbols: PLK; STPK13
• NCBI Protein Information: serine/threonine-protein kinase PLK1
• UniProt Protein Name: Serine/threonine-protein kinase PLK1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PLK1
• UniProt Synonym Gene Names: PLK; PLK-1; STPK13
• UniProt Entry Name: PLK1_HUMAN

NCBI Description

The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015]

Uniprot Description

PLK1: a kinase of the PLK family. Contains a polo-box domain (PBD), a specific phosphoserine or phosphothreonine binding domain. Substrates include BRCA2, Myt1, NudC, Cdc25C, cyclin B1, Nlp and other mitotic proteins. Inhibited by ATR. Plays a role in regulation of cytokinesis and coordinating M-phase events.

Protein type: EC 2.7.11.21; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Kinase, protein; Other group; PLK family

Chromosomal Location of Human Ortholog: 16p12.2

Cellular Component: nucleoplasm; kinetochore; microtubule cytoskeleton; spindle pole; centrosome; spindle microtubule; cytoplasm; nucleolus; spindle; spindle midzone; midbody; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; microtubule binding; kinase activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of proteolysis; regulation of cell cycle; regulation of mitotic cell cycle; mitotic nuclear envelope disassembly; protein ubiquitination; cytokinesis; anaphase-promoting complex activation during mitotic cell cycle; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; response to antibiotic; establishment of protein localization; cytokinesis after mitosis; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; centrosome organization and biogenesis; positive regulation of ubiquitin-protein ligase activity; mitotic cell cycle spindle assembly checkpoint; G2/M transition of mitotic cell cycle; mitosis; protein destabilization; mitotic sister chromatid segregation; organelle organization and biogenesis; regulation of mitotic metaphase/anaphase transition; negative regulation of cyclin-dependent protein kinase activity; cell proliferation; peptidyl-serine phosphorylation; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of protein binding; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; polar body extrusion after meiotic divisions; mitotic cell cycle; G2/M transition DNA damage checkpoint; microtubule bundle formation; mitotic metaphase/anaphase transition; negative regulation of apoptosis