Rabbit anti-Human PLEKHG3 Polyclonal Antibody | anti-PLEKHG3 antibody

PLEKHG3, CT (PLEKHG3, KIAA0599, Pleckstrin homology domain-containing family G member 3) (APC)

Cat. Num. AAA6334873

• Gene Names: PLEKHG3; ARHGEF43; KIAA0599
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PLEKHG3; Polyclonal Antibody; CT (PLEKHG3; KIAA0599; Pleckstrin homology domain-containing family G member 3) (APC); Pleckstrin homology domain-containing family G member 3; anti-PLEKHG3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PLEKHG3 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PLEKHG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1185-1214 amino acids from the C-terminal region of human PLEKHG3.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PLEKHG3 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PLEKHG3 antibody detected the PLEKHG3 protein (arrow).)

Immunohistochemistry (IHC)

(PLEKHG3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PLEKHG3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-PLEKHG3 antibody

Antibodies; Pleckstrin Homology Proteins

References

Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 55749621
• NCBI GeneID: 26030
• NCBI Accession #: NP_056364.1
• NCBI GenBank Nucleotide #: NM_015549.1
• UniProt Accession #: A1L390; O60339; Q6GMS3; Q6P4B1; Q7L3S3; Q86SW7; Q8TEF5; Q96EW6; Q9BT82; A1L389; B5MEC9
• Molecular Weight: 128,247 Da
• NCBI Official Full Name: pleckstrin homology domain-containing family G member 3
• NCBI Official Synonym Full Names: pleckstrin homology domain containing, family G (with RhoGef domain) member 3
• NCBI Official Symbol: PLEKHG3
• NCBI Official Synonym Symbols: ARHGEF43; KIAA0599
• NCBI Protein Information: pleckstrin homology domain-containing family G member 3; PH domain-containing family G member 3; pleckstrin homology domain containing, family G, member 3
• UniProt Protein Name: Pleckstrin homology domain-containing family G member 3
• Protein Family: Pleckstrin homology domain-containing family
• UniProt Gene Name: PLEKHG3
• UniProt Synonym Gene Names: KIAA0599; PH domain-containing family G member 3

Uniprot Description

PLEKHG3: 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GEF; GEF, Rac/Rho

Chromosomal Location of Human Ortholog: 14q23.3

Molecular Function: Rho guanyl-nucleotide exchange factor activity

Biological Process: positive regulation of GTPase activity; regulation of Rho protein signal transduction