Western Blot (WB)
(PLEKHA8 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PLEKHA8 antibody detected the PLEKHA8 protein (arrow).)
Rabbit anti-Human PLEKHA8 Polyclonal Antibody | anti-PLEKHA8 antibody
PLEKHA8 Antibody (C-term)
Gene Names
PLEKHA8; FAPP2
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PLEKHA8; Polyclonal Antibody; PLEKHA8 Antibody (C-term); Pleckstrin homology domain-containing family A member 8; PH domain-containing family A member 8; Phosphatidylinositol-four-phosphate adapter protein 2; FAPP-2; Phosphoinositol 4-phosphate adapter protein 2; hFAPP2; Serologically defined breast cancer antigen NY-BR-86; FAPP2; anti-PLEKHA8 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PLEKHA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 456-482 amino acids from the C-terminal region of human PLEKHA8.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
456-482
Sequence Length
519
Applicable Applications for anti-PLEKHA8 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(PLEKHA8 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PLEKHA8 antibody detected the PLEKHA8 protein (arrow).)
Western Blot (WB)
(PLEKHA8 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PLEKHA8 antibody detected the PLEKHA8 protein (arrow).)
Related Product Information for anti-PLEKHA8 antibody
PLEKHA8 is involved in TGN-to-plasma membrane transport and in the formation of post-Golgi constitutive carriers. May play a role in ensuring the coordination of the budding and the fission reactions.
Product Categories/Family for anti-PLEKHA8 antibody
References
Tsao, D.A., et al. DNA Cell Biol. 29(6):285-293(2010)
Cao, X., et al. Proc. Natl. Acad. Sci. U.S.A. 106(50):21121-21125(2009)
Tritz, R., et al. Biochem. Biophys. Res. Commun. 383(2):167-171(2009)
Yamaji, T., et al. IUBMB Life 60(8):511-518(2008)
D'Angelo, G., et al. Nature 449(7158):62-67(2007)
Cao, X., et al. Proc. Natl. Acad. Sci. U.S.A. 106(50):21121-21125(2009)
Tritz, R., et al. Biochem. Biophys. Res. Commun. 383(2):167-171(2009)
Yamaji, T., et al. IUBMB Life 60(8):511-518(2008)
D'Angelo, G., et al. Nature 449(7158):62-67(2007)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58261
NCBI Official Full Name
pleckstrin homology domain-containing family A member 8 isoform 1
NCBI Official Synonym Full Names
pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 8
NCBI Official Symbol
PLEKHA8
NCBI Official Synonym Symbols
FAPP2
NCBI Protein Information
pleckstrin homology domain-containing family A member 8
UniProt Protein Name
Pleckstrin homology domain-containing family A member 8
Protein Family
UniProt Gene Name
PLEKHA8
UniProt Synonym Gene Names
FAPP2; PH domain-containing family A member 8; FAPP-2; Phosphoinositol 4-phosphate adapter protein 2; hFAPP2
UniProt Entry Name
PKHA8_HUMAN
Uniprot Description
PLEKHA8: Cargo transport protein that is required for apical transport from the Golgi complex. Transports AQP2 from the trans- Golgi network (TGN) to sites of AQP2 phosphorylation. Mediates the non-vesicular transport of glucosylceramide (GlcCer) from the trans-Golgi network (TGN) to the plasma membrane and plays a pivotal role in the synthesis of complex glycosphingolipids. Binding of both phosphatidylinositol 4-phosphate (PIP) and ARF1 are essential for the GlcCer transfer ability. Also required for primary cilium formation, possibly by being involved in the transport of raft lipids to the apical membrane, and for membrane tubulation. 3 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 7p14.3
Cellular Component: membrane; trans-Golgi network
Molecular Function: glycolipid transporter activity; glycolipid binding
Biological Process: protein transport; lipid transport; glycolipid transport
Research Articles on PLEKHA8
Similar Products
Product Notes
The PLEKHA8 plekha8 (Catalog #AAA9207664) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 456-482. The PLEKHA8 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PLEKHA8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the PLEKHA8 plekha8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLEKHA8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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