Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PLEK antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat heart using PLEK antibody at dilution of 1:200 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human stomach using PLEK antibody at dilution of 1:200 (40x lens).)
NCBI and Uniprot Product Information
Uniprot Description
pleckstrin: Major protein kinase C substrate of platelets.
Protein type: Lipid-binding
Chromosomal Location of Human Ortholog: 2p13.3
Cellular Component: membrane; cytoplasm; extracellular region; cytosol
Molecular Function: protein binding; protein homodimerization activity; protein kinase C binding; phosphatidylinositol-3,4-bisphosphate binding
Biological Process: negative regulation of calcium-mediated signaling; integrin-mediated signaling pathway; platelet activation; phospholipase C inhibition; positive regulation of actin filament depolymerization; negative regulation of G-protein coupled receptor protein signaling pathway; positive regulation of integrin activation; platelet degranulation; cell projection organization and biogenesis; actin cytoskeleton reorganization; ruffle organization and biogenesis; positive regulation of actin filament bundle formation; hemopoietic progenitor cell differentiation; phosphatidylinositol metabolic process; cortical actin cytoskeleton organization and biogenesis; blood coagulation; vesicle docking during exocytosis
Research Articles on PLEK
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Product Notes
The PLEK plek (Catalog #AAA9125139) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PLEK reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PLEK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the PLEK plek for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLEK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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