Western Blot (WB)
(Western blot analysis of PLC gamma 1 expression in Hela (A), HepG2 (B), mouse brain (C), rat kidney (D) whole cell lysates.)
Rabbit PLC gamma 1 Polyclonal Antibody | anti-PLCG1 antibody
Anti-PLC gamma 1 Antibody
Western Blot (WB)
(Western blot analysis of PLC gamma 1 expression in Hela (A), HepG2 (B), mouse brain (C), rat kidney (D) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of PLC gamma 1 expression in Hela (A), HepG2 (B), mouse brain (C), rat kidney (D) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PLC gamma 1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PLC gamma 1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
PLCG1: a calcium dependent phosphatidylinositol-specific phospholipase C. The activated enzyme produces the second messenger molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylated and activated by tyrosine kinases in response to signaling through a variety of growth factor receptors and immune system receptors.
Protein type: EC 3.1.4.11; Phospholipase; Motility/polarity/chemotaxis; Carbohydrate Metabolism - inositol phosphate
Chromosomal Location of Human Ortholog: 20q12-q13.1
Cellular Component: cell projection; cytoplasm; cytosol; intercellular junction; lamellipodium; plasma membrane; ruffle; signalosome
Molecular Function: calcium ion binding; glutamate receptor binding; neurotrophin TRKA receptor binding; phosphoinositide phospholipase C activity; phospholipase C activity; protein binding; protein kinase binding; receptor signaling protein activity; receptor tyrosine kinase binding
Biological Process: activation of MAPKK activity; axon guidance; blood coagulation; calcium-mediated signaling; cell migration; cytokine and chemokine mediated signaling pathway; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; in utero embryonic development; innate immune response; inositol phosphate metabolic process; leukocyte migration; nerve growth factor receptor signaling pathway; phospholipase C activation; phospholipid catabolic process; positive regulation of angiogenesis; positive regulation of blood vessel endothelial cell migration; positive regulation of release of sequestered calcium ion into cytosol; signal transduction; T cell receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway; viral reproduction
Research Articles on PLCG1
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Product Notes
The PLCG1 plcg1 (Catalog #AAA8242782) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PLC gamma 1 Antibody reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's PLC gamma 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200). Researchers should empirically determine the suitability of the PLCG1 plcg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLC gamma 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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