Rabbit anti-Human PLAUR Polyclonal Antibody | anti-PLAUR antibody

PLAUR, ID (PLAUR, MO3, UPAR, Urokinase plasminogen activator surface receptor, Monocyte activation antigen Mo3, CD87) (AP)

Cat. Num. AAA6334630

• Gene Names: PLAUR; CD87; UPAR; URKR; U-PAR
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PLAUR; Polyclonal Antibody; ID (PLAUR; MO3; UPAR; Urokinase plasminogen activator surface receptor; Monocyte activation antigen Mo3; CD87) (AP); CD87; anti-PLAUR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-PLAUR antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: PLAUR antibody is generated from rabbits immunized with a KLH conjugated Synthetic peptide selected from the center region of human PLAUR.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(Western blot analysis of PLAUR (arrow) using rabbit polyclonal PLAUR Antibody (W151). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PLAUR gene.)

Western Blot (WB)

(Western blot analysis of PLAUR (arrow) using rabbit polyclonal PLAUR Antibody (W151). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PLAUR gene.)

Related Product Information for anti-PLAUR antibody

The urokinase-type plasminogen activator receptor is a key molecule in the regulation of cell-surface plasminogen activation and plays an important role in many normal as well as pathologic processes. The human PLAUR cDNA encodes 335 amino acids including a predicted signal peptide of 22 residues and a hydrophobic C-terminal portion.1 It produces a highly glycosylated protein of about 50 kD in monocytes where it is anchored to the plasma membrane by glycosyl-phosphatidylinositol linkage. PLAUR, also known as UPAR, is directly associated with the carbohydrate-binding domain of SELL in the membrane of neutrophils, an association analogous to that between PLAUR and beta-2 integrins.2 PLAUR-mediated calcium mobilization is SELL dependent. UPAR mRNA levels correlate with the invasive potential of endometrial carcinomas and show a 33-fold increase in UPAR mRNA levels in advanced clinical stage endometrial tumors compared with normal endometrial tissue.3 Furthermore, the increase in UPAR mRNA levels correlated linearly with the progression of disease stage. UPAR protein expressioin correlated positively with rate of recurrence and mortality in patients with endometrial cancer.4 UPAR appears to be a useful prognostic marker for advanced endometrial cancer.

Product Categories/Family for anti-PLAUR antibody

Antibodies; CD Markers

References

Borgfeldt, C., et al., Int. J. Cancer 107(4):658-665 (2003). Tran, H., et al., Mol. Cell. Biol. 23(20):7177-7188 (2003). Coleman, J.L., et al., Infect. Immun. 71(10):5556-5564 (2003). Sturge, J., et al., J. Cell Biol. 162(5):789-794 (2003). Li, Y., et al., J. Biol. Chem. 278(32):29925-29932 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4505865
• NCBI GeneID: 5329
• NCBI Accession #: NP_002650.1
• NCBI GenBank Nucleotide #: NM_002659.3
• UniProt Accession #: Q03405; Q12876; Q15845; Q16887; Q6IB52; Q9BWT0; Q9NYC8; Q9UD69; Q9UEA6; Q9UM92; Q9UMV0; A8K409
• Molecular Weight: 36,978 Da
• NCBI Official Full Name: urokinase plasminogen activator surface receptor isoform 1
• NCBI Official Synonym Full Names: plasminogen activator, urokinase receptor
• NCBI Official Symbol: PLAUR
• NCBI Official Synonym Symbols: CD87; UPAR; URKR; U-PAR
• NCBI Protein Information: urokinase plasminogen activator surface receptor; monocyte activation antigen Mo3; u-plasminogen activator receptor form 2; urokinase-type plasminogen activator (uPA) receptor
• UniProt Protein Name: Urokinase plasminogen activator surface receptor
• Protein Family: Urokinase plasminogen activator surface receptor
• UniProt Gene Name: PLAUR
• UniProt Synonym Gene Names: MO3; UPAR; U-PAR; uPAR
• UniProt Entry Name: UPAR_HUMAN

NCBI Description

This gene encodes the receptor for urokinase plasminogen activator and, given its role in localizing and promoting plasmin formation, likely influences many normal and pathological processes related to cell-surface plasminogen activation and localized degradation of the extracellular matrix. It binds both the proprotein and mature forms of urokinase plasminogen activator and permits the activation of the receptor-bound pro-enzyme by plasmin. The protein lacks transmembrane or cytoplasmic domains and may be anchored to the plasma membrane by a glycosyl-phosphatidylinositol (GPI) moiety following cleavage of the nascent polypeptide near its carboxy-terminus. However, a soluble protein is also produced in some cell types. Alternative splicing results in multiple transcript variants encoding different isoforms. The proprotein experiences several post-translational cleavage reactions that have not yet been fully defined. [provided by RefSeq, Jul 2008]

Uniprot Description

uPAR: Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form. Monomer (Probable). Interacts with MRC2. Interacts (via the UPAR/Ly6 domains) with SRPX2. Expressed in neurons of the rolandic area of the brain. Expressed in the brain. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, GPI anchor; Receptor, misc.

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: endoplasmic reticulum membrane; extrinsic to membrane; focal adhesion; integral to plasma membrane; endoplasmic reticulum lumen; integral to membrane; plasma membrane

Molecular Function: protein domain specific binding; protein binding; U-plasminogen activator receptor activity; enzyme binding; receptor activity; receptor binding

Biological Process: fibrinolysis; cellular protein metabolic process; attachment of GPI anchor to protein; regulation of proteolysis; C-terminal protein lipidation; chemotaxis; post-translational protein modification; cell motility; blood coagulation; signal transduction