Sheep Platelet Factor 4 Polyclonal Antibody | anti-PF4 antibody

Sheep anti-human Platelet Factor 4 (PF4), Whole IgG from Antiserum

Cat. Num. AAA512119

• Gene Names: PF4; CXCL4; SCYB4; MGC138298
• Purity: Purified IgG.; Vial containing ml of whole IgG representing approximately 1 ml of antiserum. Total protein is 10 mg.
• Synonyms: Platelet Factor 4; Polyclonal Antibody; Sheep anti-human Platelet Factor 4 (PF4); Whole IgG from Antiserum; human; anti-PF4 antibody

• Host: Sheep
• Clonality: Polyclonal
• Specificity: This antibody is specific for Platelet Factor 4 as demonstrated by immunoelectrophoresis and ELISA.
• Purity/Purification: Purified IgG.; Vial containing ml of whole IgG representing approximately 1 ml of antiserum. Total protein is 10 mg.
• Form/Format: Whole IgG, clear liquid
• Concentration: IgG concentration is mg/ml, determined by absorbance using an extinction coefficient (E1%280) of 13.4. (varies by lot)

• Application Notes: Suitable as a source of antibodies to human Platelet Factor 4.
• Immunogen: Platelet Factor 4 purified from human platelet releasate.
• Buffer: 10 mM HEPES, pH 7.4, 150 mM NaCl, 50% (v/v) glycerol
• Neutralizing Activity: Not determined
• Preparation and Storage: Store between -10 and -20 degree C. Product will become viscous but will not freeze. Avoid storage in frost-free freezers. Keep vial tightly capped. Allow product to warm to room temperature and gently mix before use.

Related Product Information for anti-PF4 antibody

Platelet Factor 4 (PF4) is a small high-affinity heparin binding protein localized in the alpha-granules of platelets and megacaryocytes. It is a member of a multigene "intercrinecytokine" family that includes beta-thromboglobulin, connective tissue activating peptide III, monocyte-derived neutrophil chemotactic factor and melanoma growth stimulatory factor. Human PF4 consists of 70 amino acids and has a mass of 7800 daltons. In the platelet, PF4 exists as a homo-tetramer in complex with a high-molecular weight proteoglycan carrier. PF4 is one of the most abundant proteins in the platelet alpha-granule and is considered platelet-specific, as its concentration in platelets is more than 20,000-fold greater than in plasma. The heparin neutralization activity of PF4 is believed to be due to the presence of four lysine residues interspersed among pairs of aliphatic residues in the C-terminal region of the molecule. During vessel injury, platelets are stimulated by thrombin or other agonists to release alpha-granule contents at the site of injury. Presumably the potent heparin neutralization activity of PF4 would reduce the anticoagulant activity of antithrombin, prolonging the action of thrombin and activated factor X to promote clot formation. Released PF4 also binds to heparin-like molecules on the surface of endothelial cells and hepatocytes, neutralizing the anticoagulant activity expressed by these cells. Some of the other activities attributable to PF4 include the potentiation of platelet aggregation, stimulation of fibroblast attachment to substrata, chemotactic activity with respect to neutrophils and inhibition of contact activation1,2.

References

1. Rucinski B, Niewiarowski S, Strzyzewski M, Holt JC, Mayo KH; Human Platelet Factor 4 and its C-Terminal Peptides: Heparin Binding and Clearance from the Circulation; Thrombosis and Haemostasis 63, pp 493-498, 1990.
2. Biochemistry and Physiology of Secreted Platelet Proteins; in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 546-566, J.B. Lippincott Co., Philadelphia PA, USA, 1994.

NCBI and Uniprot Product Information

• NCBI GI #: 4505733
• NCBI GeneID: 5196
• NCBI Accession #: NP_002610.1
• NCBI GenBank Nucleotide #: NM_002619.2
• UniProt Accession #: P02776; Q53X61; Q9UC64; Q9UC65
• Molecular Weight: 10714 Da
• NCBI Official Full Name: platelet factor 4
• NCBI Official Synonym Full Names: platelet factor 4
• NCBI Official Symbol: PF4
• NCBI Official Synonym Symbols: CXCL4; SCYB4; MGC138298
• NCBI Protein Information: platelet factor 4; PF-4; iroplact; oncostatin-A; OTTHUMP00000160478; C-X-C motif chemokine 4; chemokine (C-X-C motif) ligand 4
• UniProt Protein Name: Platelet factor 4
• Protein Family: Platelet factor
• UniProt Gene Name: PF4
• UniProt Synonym Gene Names: CXCL4; SCYB4
• UniProt Entry Name: PLF4_HUMAN

NCBI Description

Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair (Eisman et al., 1990 [PubMed 1695112]).[supplied by OMIM]

Uniprot Description

Function: Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. Ref.11

Subunit structure: Homotetramer.

Subcellular location: Secreted.

Post-translational modification: Binds non-covalently to a proteoglycan molecule.

Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.

Mass spectrometry: Molecular mass is 7765 Da from positions 32 - 101. Determined by ESI. Ref.11Molecular mass is 6033 Da from positions 48 - 101. Determined by ESI. Short form. Ref.11