Rabbit anti-Human Plasminogen Polyclonal Antibody | anti-PLG antibody

Plasminogen, CT (PLG) (HRP)

Cat. Num. AAA6493694

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation and Protein A/G Column Purification.
• Synonyms: Plasminogen; Polyclonal Antibody; CT (PLG) (HRP); anti-PLG antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PLG.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation and Protein A/G Column Purification.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PLG antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: IHC-P: 1:10-1:50; ELISA: 1:1,000; WB: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 636-655 near the C-terminal region of human PLG, UniProt accession #P00747.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PLG in K562 cell line lysate (35ug/lane) usingMBS6013519.)

Immunohistochemistry (IHC)

(IHC staining of formalin-fixed and paraffin-embedded human hepatocarcinoma usingMBS6013519. Peroxidase-conjugated secondary antibody and DAB staining were used for visualization of the antigen. )

Flow Cytometry (FC/FACS)

(Fow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibody was used for the analysis.)

Product Categories/Family for anti-PLG antibody

Antibodies; Coagulation Factors

References

1. Hofmann, S.C., Voith, U. J. Invest. Dermatol. 129(7), 1730-1739 (2009). 2. Passero, C.J., Mueller, G.M. J. Biol. Chem. 283(52), 36586-36591 (2008). 3. Ohyama, S., Harada, T. Eur. J. Biochem. 271(4), 809-820 (2004). 4. Lee, H., Kim, H.K. Arch. Biochem. Biophys. 375(2), 359-363 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 4505881
• NCBI GeneID: 5340
• NCBI Accession #: NP_000292
• NCBI GenBank Nucleotide #: NM_000301
• UniProt Accession #: P00747; Q15146; Q5TEH4; Q6PA00
• Molecular Weight: 90,569 Da
• NCBI Official Full Name: plasminogen isoform 1
• NCBI Official Synonym Full Names: plasminogen
• NCBI Official Symbol: PLG
• NCBI Protein Information: plasminogen
• UniProt Protein Name: Plasminogen
• Protein Family: Plasminogen
• UniProt Gene Name: PLG
• UniProt Entry Name: PLMN_HUMAN

Uniprot Description

Plasminogen: Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells. Defects in PLG are the cause of plasminogen deficiency (PLGD). PLGD is characterized by decreased serum plasminogen activity. Two forms of the disorder are distinguished: type 1 deficiency is additionally characterized by decreased plasminogen antigen levels and clinical symptoms, whereas type 2 deficiency, also known as dysplasminogenemia, is characterized by normal, or slightly reduced antigen levels, and absence of clinical manifestations. Plasminogen deficiency type 1 results in markedly impaired extracellular fibrinolysis and chronic mucosal pseudomembranous lesions due to subepithelial fibrin deposition and inflammation. The most common clinical manifestation of type 1 deficiency is ligneous conjunctivitis in which pseudomembranes formation on the palpebral surfaces of the eye progresses to white, yellow-white, or red thick masses with a wood-like consistency that replace the normal mucosa. Belongs to the peptidase S1 family. Plasminogen subfamily.

Protein type: Secreted; Secreted, signal peptide; Protease; Motility/polarity/chemotaxis; EC 3.4.21.7

Chromosomal Location of Human Ortholog: 6q26

Cellular Component: extracellular space; cell surface; extrinsic to external side of plasma membrane; extracellular region; plasma membrane

Molecular Function: protein domain specific binding; protein binding; serine-type peptidase activity; serine-type endopeptidase activity; apolipoprotein binding; receptor binding

Biological Process: platelet activation; extracellular matrix organization and biogenesis; tissue remodeling; myoblast differentiation; muscle maintenance; negative regulation of cell proliferation; extracellular matrix disassembly; fibrinolysis; platelet degranulation; cellular protein metabolic process; negative regulation of fibrinolysis; proteolysis involved in cellular protein catabolic process; tissue regeneration; positive regulation of fibrinolysis; blood coagulation; transmembrane transport

Disease: Plasminogen Deficiency, Type I