Western Blot (WB)
(MBS422392 (1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
Goat PLA2G4A Polyclonal Antibody | anti-PLA2G4A antibody
Goat anti-PLA2G4A Antibody
Western blot: Approx 80-85kDa band observed in lysates of cell line A549 (calculated MW of 85.2kDa according to NP_077734.1). Preliminary experiments also showed an 85- 90kDa band in lysates of cell lines A431, HeLa and NIH-3T3 Recommended concentration:0.3-1ug/ml. Primary incubation 1 hour at room temperature.Preliminary testing was unsuccessful on Rat for this particular batch.
IHC: Paraffin embedded Human Uterus. Recommended concentration: 3.75ug/ml.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A549 cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.
Western Blot (WB)
(MBS422392 (1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
Western Blot (WB)
(MBS422392 (1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
Uniprot Description
cPLA2: a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Arachidonic acid is a precursor for the eicosanoids that are involved in hemodynamic regulation, inflammatory responses, and other cellular processes. Promotes cerebellar long-term depression and motor learning. Translocates to the Golgi and endoplasmic reticulum in a calcium-dependent fashion. Translocation and activation of at the ER facilitates the process of ER stress. Regulates the biogenesis of lipid droplets, a process dependent on JNK and ceramide kinase. Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+. The N-terminal C2 domain, by its association with lipid membranes, mediates the regulation of CPLA2 by presenting the active site to its substrate in response to elevations of cytosolic Ca2+. Inhibited when in trimolecular complex with ANXA2 and S100A10. Phosphorylation of S727 relieves this inhibitory interaction, thus activating PLA2G4A.
Protein type: EC 3.1.1.4; EC 3.1.1.5; Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - arachidonic acid; Lipid Metabolism - ether lipid; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - linoleic acid; Phospholipase
Chromosomal Location of Human Ortholog: 1q25
Cellular Component: cytoplasm; cytosol; endoplasmic reticulum membrane; mitochondrial inner membrane
Molecular Function: calcium ion binding; calcium-dependent phospholipase A2 activity; calcium-dependent phospholipid binding; lysophospholipase activity; phospholipase A1 activity; phospholipase A2 activity
Biological Process: arachidonic acid metabolic process; phosphatidic acid biosynthetic process; phospholipid metabolic process
Research Articles on PLA2G4A
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Product Notes
The PLA2G4A pla2g4a (Catalog #AAA422392) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PLA2G4A Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PLA2G4A can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA, Western Blot (WB), Immunohhistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 80-85kDa band observed in lysates of cell line A549 (calculated MW of 85.2kDa according to NP_077734.1). Preliminary experiments also showed an 85- 90kDa band in lysates of cell lines A431, HeLa and NIH-3T3 Recommended concentration:0.3-1ug/ml. Primary incubation 1 hour at room temperature.Preliminary testing was unsuccessful on Rat for this particular batch. IHC: Paraffin embedded Human Uterus. Recommended concentration: 3.75ug/ml. Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A549 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml. Researchers should empirically determine the suitability of the PLA2G4A pla2g4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QEKTFRQQRK EHIR. It is sometimes possible for the material contained within the vial of "PLA2G4A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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