Rabbit anti-Human PLA2G3 Polyclonal Antibody | anti-PLA2G3 antibody

PLA2G3 Antibody

Cat. Num. AAA9630135

• Gene Names: PLA2G3; SPLA2III; sPLA2-III; GIII-SPLA2
• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: PLA2G3; Polyclonal Antibody; PLA2G3 Antibody; EC 3.1.1.4; GIII sPLA2; Group 3 secretory phospholipase A2; Group III secretory phospholipase A2; PA2G3_HUMAN; Phosphatidylcholine 2 acylhydrolase GIII; Phosphatidylcholine 2-acylhydrolase 3; phospholipase A2 group III; sPLA2-III; SPLA2III; anti-PLA2G3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: PLA2G3 Antibody detects endogenous levels of PLA2G3.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PLA2G3 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human PLA2G3.
• Conjugation: Unconjugated
• Similarity: Belongs to the phospholipase A2 family.
• Post Translational Modifications: N-glycosylation does not affect the catalytic activity, but is required for proper secretion. A nonglycosylated form was observed in several cell types.In several cell types, the N- and C-termini are cleaved off.
• Tissue Specificity: Expressed in kidney, heart, liver, and skeletal muscle. Also present in placenta and peripheral blood leukocytes. Not detected in brain, colon, thymus, spleen and small intestine. In lung, expressed in bronchial epithelial cells and alveolar macrophages, but scarcely detected in alveolar epithelium, arterial walls and interstitial fibroblasts (at protein level). In joints of osteoarthritis and rheumatoid arthritis, expressed in endothelial cells (at protein level). In normal heart, detected in some vessels. In myocardial tissues with acute infarction, expressed in vascular endothelial cells adjacent to cardiomyocytes and those in lesions with granulation. Expression in cardiomyocytes is scarce (at protein level). In uterus, breast and colon cancers, detected in tumor cells and neighboring microvascular endothelium, but not in normal glandular tissues (at protein level).
• Function: PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Shows an 11-fold preference for phosphatidylglycerol over phosphatidylcholine (PC). Preferential cleavage: 1-palmitoyl-2-linoleoyl-phosphatidylethanolamine (PE) > 1-palmitoyl-2-linoleoyl-PC > 1-palmitoyl-2-arachidonoyl-PC > 1-palmitoyl-2-arachidonoyl-PE. Plays a role in ciliogenesis.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-PLA2G3 antibody

Secreted; Cell membrane; Cytoplasm; Cytoskeleton; Microtubule organizing center; Centrosome; Centriole; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 142976884
• NCBI GeneID: 50487
• NCBI Accession #: NP_056530.2
• NCBI GenBank Nucleotide #: NM_015715.4
• UniProt Accession #: Q9NZ20; O95768
• Molecular Weight: 57,167 Da
• NCBI Official Full Name: group 3 secretory phospholipase A2
• NCBI Official Synonym Full Names: phospholipase A2, group III
• NCBI Official Symbol: PLA2G3
• NCBI Official Synonym Symbols: SPLA2III; sPLA2-III; GIII-SPLA2
• NCBI Protein Information: group 3 secretory phospholipase A2; group III secreted phospholipase A2; phosphatidylcholine 2-acylhydrolase 3; phosphatidylcholine 2-acylhydrolase GIII
• UniProt Protein Name: Group 3 secretory phospholipase A2
• Protein Family: Group 3 secretory phospholipase
• UniProt Gene Name: PLA2G3
• UniProt Synonym Gene Names: GIII sPLA2; sPLA2-III
• UniProt Entry Name: PA2G3_HUMAN

NCBI Description

This gene encodes a protein that belongs to the secreted phospholipase A2 family, whose members include the bee venom enzyme. The encoded enzyme functions in lipid metabolism and catalyzes the calcium-dependent hydrolysis of the sn-2 acyl bond of phospholipids to release arachidonic acid and lysophospholipids. This enzyme acts as a negative regulator of ciliogenesis, and may play a role in cancer development by stimulating tumor cell growth and angiogenesis. This gene is associated with oxidative stress, and polymorphisms in this gene are linked to risk for Alzheimer's disease. [provided by RefSeq, Apr 2014]

Uniprot Description

PLA2G3: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Shows an 11-fold preference for phosphatidylglycerol over phosphatidylcholine (PC). Preferential cleavage: 1-palmitoyl-2-linoleoyl- phosphatidylethanolamine (PE) > 1-palmitoyl-2-linoleoyl-PC > 1- palmitoyl-2-arachidonoyl-PC > 1-palmitoyl-2-arachidonoyl-PE. Plays a role in ciliogenesis. Belongs to the phospholipase A2 family.

Protein type: Lipid Metabolism - arachidonic acid; EC 3.1.1.4; Lipid Metabolism - linoleic acid; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - ether lipid; Phospholipase; Lipid Metabolism - alpha-linolenic acid

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: centriole; extracellular space; mast cell granule; extracellular region; plasma membrane

Molecular Function: calcium-dependent phospholipase A2 activity; calcium ion binding

Biological Process: acrosome formation; lipoxygenase pathway; phospholipid metabolic process; glycerophospholipid biosynthetic process; sperm axoneme assembly; mast cell degranulation; lipid catabolic process