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Immunohistochemistry (IHC) (Anti-PLA2G16 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Goat PLA2G16 / HRASLS3 Polyclonal Antibody | anti-PLA2G16 antibody

Goat Polyclonal to Human PLA2G16 / HRASLS3

Gene Names
PLA2G16; AdPLA; HRSL3; HRASLS3; HREV107; HREV107-1; HREV107-3; H-REV107-1
Reactivity
Gibbon, Gorilla, Human, Monkey, Orangutan, Rabbit
Predicted Reactivity: Mouse (at least 90% immunogen sequence identity)
Applications
Immunohistochemistry, ELISA
Purity
Immunoaffinity Purified
Synonyms
PLA2G16 / HRASLS3; Polyclonal Antibody; Goat Polyclonal to Human PLA2G16 / HRASLS3; Anti-PLA2G16 / HRASLS3 Antibody (N-Terminus) IHC-plus; PLA2G16; AdPLA; Adipose-specific PLA2; Group XVI phospholipase A1/A2; Group XVI phospholipase A2; H-REV107-1; HRAS-like suppressor 3; HRASLS3; HREV107-3; HRAS-like suppressor 1; HREV107-1; H-rev 107 protein homolog; HREV107; HRSL3; Phospholipase A2; Human PLA2G16; anti-PLA2G16 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Gibbon, Gorilla, Human, Monkey, Orangutan, Rabbit
Predicted Reactivity: Mouse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Specificity
Human PLA2G16. Reported variants represent identical protein:NP_009000.2 and NP_001121675.1).
Purity/Purification
Immunoaffinity Purified
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Concentration
0.5 mg/ml (varies by lot)
Sequence Length
162
Applicable Applications for anti-PLA2G16 antibody
Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
Application Notes
ELISA (1:128000), IHC-P (5 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
Target Species
Human
Immunogen Description
Synthetic peptide RAPIPEPKPGDLIE-C from the N-terminus of human PLA2G16 (NP_009000.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Rabbit (100%); Mouse (93%); Monkey, Rat, Bovine (86%).
Immunogen Type
Synthetic peptide
Immunogen
PLA2G16 / HRASLS3 antibody was raised against synthetic peptide RAPIPEPKPGDLIE-C from the N-terminus of human PLA2G16 (NP_009000.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Rabbit (100%); Mouse (93%); Monkey, Rat, Bovine (86%).
Antigen Modification
N-Terminus
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-PLA2G16 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC) (Anti-PLA2G16 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17,937 Da
NCBI Official Full Name
HRAS-like suppressor 3
NCBI Official Synonym Full Names
phospholipase A2, group XVI
NCBI Official Symbol
PLA2G16
NCBI Official Synonym Symbols
AdPLA; HRSL3; HRASLS3; HREV107; HREV107-1; HREV107-3; H-REV107-1
NCBI Protein Information
HRAS-like suppressor 3; adipose-specific PLA2; HRAS-like suppressor 1; H-rev 107 protein homolog; group XVI phospholipase A2; group XVI phospholipase A1/A2; Ca-independent phospholipase A1/2; adipose-specific phospholipase A2; renal carcinoma antigen NY-REN-65
UniProt Protein Name
HRAS-like suppressor 3
Protein Family
UniProt Gene Name
PLA2G16
UniProt Synonym Gene Names
HRASLS3; HREV107; HRSL3; AdPLA
UniProt Entry Name
HRSL3_HUMAN

Uniprot Description

PLA2G16: Exhibits PLA1/2 activity, catalyzing the calcium- independent hydrolysis of acyl groups in various phosphotidylcholines (PC) and phosphatidylethanolamine (PE). For most substrates, PLA1 activity is much higher than PLA2 activity. Specifically catalyzes the release of fatty acids from phospholipids in adipose tissue. N- and O- acylation activity is hardly detectable. Might decrease protein phosphatase 2A (PP2A) activity. Belongs to the H-rev107 family.

Protein type: EC 3.1.1.4; Membrane protein, integral; EC 3.1.1.32

Chromosomal Location of Human Ortholog: 11q12.3

Cellular Component: perinuclear region of cytoplasm; endoplasmic reticulum; integral to membrane; cytosol

Molecular Function: phospholipase A2 activity; protein binding; phospholipase A1 activity

Biological Process: phospholipid metabolic process; glycerophospholipid biosynthetic process; lipid catabolic process; negative regulation of cell cycle

Research Articles on PLA2G16

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Product Notes

The PLA2G16 pla2g16 (Catalog #AAA242532) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Polyclonal to Human PLA2G16 / HRASLS3 reacts with Gibbon, Gorilla, Human, Monkey, Orangutan, Rabbit Predicted Reactivity: Mouse (at least 90% immunogen sequence identity) and may cross-react with other species as described in the data sheet. AAA Biotech's PLA2G16 / HRASLS3 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), ELISA (EIA). ELISA (1:128000), IHC-P (5 ug/ml) Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.. Researchers should empirically determine the suitability of the PLA2G16 pla2g16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLA2G16 / HRASLS3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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