Goat PLA2G16 Polyclonal Antibody | anti-PLA2G16 antibody

PLA2G16 (Group XVI Phospholipase A2, Adipose-specific Phospholipase A2, AdPLA, H-rev 107 Protein Homolog, HRAS-like Suppressor 3, HREV107, HREV107-3, Renal Carcinoma Antigen NY-REN-65, HRASLS3)

Cat. Num. AAA645591

• Gene Names: PLA2G16; AdPLA; HRSL3; HRASLS3; HREV107; HREV107-1; HREV107-3; H-REV107-1
• Reactivity: Bovine, Human, Mouse, Rat
• Applications: ELISA
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: PLA2G16; Polyclonal Antibody; PLA2G16 (Group XVI Phospholipase A2; Adipose-specific Phospholipase A2; AdPLA; H-rev 107 Protein Homolog; HRAS-like Suppressor 3; HREV107; HREV107-3; Renal Carcinoma Antigen NY-REN-65; HRASLS3); Anti -PLA2G16 (Group XVI Phospholipase A2; anti-PLA2G16 antibody

• Host: Goat
• Reactivity: Bovine, Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes PLA2G16. Species sequence homology: Bovine, human, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

• Applicable Applications for anti-PLA2G16 antibody: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA.; Dilution: ELISA: 1:128,000
• Immunogen: Synthetic peptide corresponding to RAPIPEPKPGDLIE-C, from PLA2G116, at N-terminal (NP_009000.2).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-PLA2G16 antibody

Exhibits PLA1/2 activity, catalyzing the calcium-independent hydrolysis of acyl groups in various phosphotidylcholines (PC) and phosphatidylethanolamine (PE). For most substrates, PLA1 activity is much higher than PLA2 activity. Specifically catalyzes the release of fatty acids from phospholipids in adipose tissue By similarity. N- and O-acylation activity is hardly detectable. Might decrease protein phosphatase 2A (PP2A) activity.

Product Categories/Family for anti-PLA2G16 antibody

Antibodies; Abs to Enzymes; Phospholipase

NCBI and Uniprot Product Information

• NCBI GI #: 75517991
• NCBI GeneID: 11145
• UniProt Accession #: P53816; Q3SYI3; Q9HDD1; B2R7Q4; B7XAK5
• Molecular Weight: 17,937 Da
• NCBI Official Full Name: PLA2G16 protein
• NCBI Official Synonym Full Names: phospholipase A2, group XVI
• NCBI Official Symbol: PLA2G16
• NCBI Official Synonym Symbols: AdPLA; HRSL3; HRASLS3; HREV107; HREV107-1; HREV107-3; H-REV107-1
• NCBI Protein Information: HRAS-like suppressor 3; adipose-specific PLA2; HRAS-like suppressor 1; H-rev 107 protein homolog; group XVI phospholipase A2; group XVI phospholipase A1/A2; Ca-independent phospholipase A1/2; adipose-specific phospholipase A2; renal carcinoma antigen NY-REN-65
• UniProt Protein Name: HRAS-like suppressor 3
• Protein Family: HRAS-like suppressor
• UniProt Gene Name: PLA2G16
• UniProt Synonym Gene Names: HRASLS3; HREV107; HRSL3; AdPLA
• UniProt Entry Name: HRSL3_HUMAN

Uniprot Description

Function: Exhibits PLA1/2 activity, catalyzing the calcium-independent hydrolysis of acyl groups in various phosphatidylcholines (PC) and phosphatidylethanolamine (PE). For most substrates, PLA1 activity is much higher than PLA2 activity. Specifically catalyzes the release of fatty acids from phospholipids in adipose tissue

By similarity. N- and O-acylation activity is hardly detectable. Might decrease protein phosphatase 2A (PP2A) activity. Ref.4 Ref.10

Catalytic activity: Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate. Ref.12 Ref.13Phosphatidylcholine + H2O = 2-acylglycerophosphocholine + a carboxylate. Ref.12 Ref.13

Subunit structure: Interacts with PPP2R1A; this interaction might decrease PP2A activity. Ref.10

Subcellular location: Cytoplasm. Cytoplasm › perinuclear region

By similarity. Membrane; Single-pass membrane protein Ref.10 Ref.12.

Tissue specificity: Widely expressed. low expression, if any, in hematopoietic cells and thymus. In testis, confined to round spermatids. Expressed in normal ovarian epithelial cells. Down-regulated in some ovarian carcinomas and testicular germ cell tumors. Ref.1 Ref.3 Ref.4 Ref.9

Induction: By IFNG and IRF1. Ref.9

Sequence similarities: Belongs to the H-rev107 family.

Biophysicochemical propertiesKinetic parameters:KM=300 µM for dipalmitoyl-PC Ref.4Vmax=2.57 µmol/min/mg enzyme with dipalmitoyl-PC as substrateVmax=267 nmol/min/mg enzyme with dipalmitoyl-PE as substratepH dependence:Optimum pH is 9.