Rabbit anti-Human PKR Polyclonal Antibody | anti-PKR antibody

PKR (Ab-451) Antibody

Cat. Num. AAA9402485

• Gene Names: EIF2AK2; PKR; PRKR; EIF2AK1; PPP1R83
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• Synonyms: PKR; Polyclonal Antibody; PKR (Ab-451) Antibody; ADRB2; E2AK2; EIF2AK2; EIF2aK; PRKR; anti-PKR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total PKR protein.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 551

• Applicable Applications for anti-PKR antibody: Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Immunohistochemistry: 1:50~1:100; Immunofluorescence: 1:100~1:200
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around aa.449~453 (K-G-T-L-R) derived from Human PKR.
• Target Name: PKR
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PKR(Ab-451) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed Hela cells using PKR(Ab-451) Antibody.)

Related Product Information for anti-PKR antibody

Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.

Product Categories/Family for anti-PKR antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 208431827
• NCBI GeneID: 5610
• NCBI Accession #: NP_001129123.1
• NCBI GenBank Nucleotide #: NM_001135651.2
• UniProt Accession #: P19525; Q52M43; Q7Z6F6; Q9UIR4; A8K3P0; D6W584; E9PC80
• Molecular Weight: 57,391 Da
• NCBI Official Full Name: interferon-induced, double-stranded RNA-activated protein kinase isoform a
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2-alpha kinase 2
• NCBI Official Symbol: EIF2AK2
• NCBI Official Synonym Symbols: PKR; PRKR; EIF2AK1; PPP1R83
• NCBI Protein Information: interferon-induced, double-stranded RNA-activated protein kinase
• UniProt Protein Name: Interferon-induced, double-stranded RNA-activated protein kinase
• UniProt Gene Name: EIF2AK2
• UniProt Synonym Gene Names: PKR; PRKR; eIF-2A protein kinase 2; PKR; Protein kinase R
• UniProt Entry Name: E2AK2_HUMAN

NCBI Description

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

PKR: a protein kinase of the PEK family. Upon binding double-stranded RNA, it becomes autophosphorylated and activated. Phosphorylates and inhibits the alpha subunit of eIF2 alpha, which leads to an inhibition of the initiation of protein synthesis. Controls the activation of several transcription factors such as NF-kappaB, p53 and Stats. Mediates apoptosis induced by many different stimuli, such as LPS, TNF-alpha, viral infection and serum starvation.

Protein type: Protein kinase, Other; EC 2.7.10.2; Translation; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Other group; PEK family

Chromosomal Location of Human Ortholog: 2p22-p21

Cellular Component: membrane; perinuclear region of cytoplasm; cytoplasm; ribosome; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; double-stranded RNA binding; non-membrane spanning protein tyrosine kinase activity; protein phosphatase type 2A regulator activity; ATP binding; eukaryotic translation initiation factor 2alpha kinase activity; protein kinase activity

Biological Process: positive regulation of cytokine production; peptidyl-tyrosine phosphorylation; activation of MAPKK activity; translation; transcription, DNA-dependent; unfolded protein response; response to virus; protein amino acid autophosphorylation; viral infectious cycle; protein amino acid phosphorylation; positive regulation of chemokine production; evasion by virus of host immune response; positive regulation of stress-activated MAPK cascade; activation of NF-kappaB transcription factor; negative regulation of cell proliferation; modification by virus of host cellular process; negative regulation of viral genome replication; virus-host interaction; negative regulation of translation; innate immune response; negative regulation of osteoblast proliferation; defense response to virus; negative regulation of apoptosis