Rabbit PKM / Pyruvate Kinase Polyclonal Antibody | anti-PKM antibody

Rabbit Polyclonal (IgG) to Human PKM / Pyruvate Kinase

Cat. Num. AAA242047

• Gene Names: PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: PKM / Pyruvate Kinase; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human PKM / Pyruvate Kinase; Anti-PKM / Pyruvate Kinase Antibody IHC-plus; PKM; p58; PK3; PKM2; Pyruvate Kinase; Pyruvate kinase 2/3; Pyruvate kinase; m1; muscle; TCB; Tumor M2-PK; OIP-3; OIP3; Opa-interacting protein 3; CTHBP; PK; muscle type; PK2; Pyruvate kinase isozymes M1/M2; Human PKM; anti-PKM antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous levels of total human PKM2 protein at 60kD. Species cross-reactivity: monkey, mouse and rat.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 100 ug/ml BSA, 50% glycerol.
• Sequence Length: 531

• Applicable Applications for anti-PKM antibody: Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Western Blot (WB)
• Application Notes: IHC-Fr (1:100), IHC-P (1:100), WB (1:1000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide derived from the sequence of human PKM2 (KLH). Molecular Weight: 60kD.
• Immunogen Type: Synthetic peptide
• Immunogen: PKM / Pyruvate Kinase antibody was raised against synthetic peptide derived from the sequence of human PKM2 (KLH). Molecular Weight: 60kD.
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-PKM2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Related Product Information for anti-PKM antibody

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones.

NCBI and Uniprot Product Information

• NCBI GI #: 33286418
• NCBI GeneID: 5315
• NCBI Accession #: NP_002645.3
• NCBI GenBank Nucleotide #: NM_002654.4
• UniProt Accession #: P14618; P14786; Q53GK4; Q96E76; Q9BWB5; Q9UCV6; Q9UPF2; A6NFK3; B2R5N8; B3KRY0; B4DFX8; B4DUU6
• Molecular Weight: 56,273 Da
• NCBI Official Full Name: pyruvate kinase PKM isoform a
• NCBI Official Synonym Full Names: pyruvate kinase, muscle
• NCBI Official Symbol: PKM
• NCBI Official Synonym Symbols: PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
• NCBI Protein Information: pyruvate kinase PKM; p58; OIP-3; tumor M2-PK; PK, muscle type; pyruvate kinase 2/3; OPA-interacting protein 3; pyruvate kinase isozymes M1/M2; pyruvate kinase muscle isozyme; thyroid hormone-binding protein 1; epididymis secretory protein Li 30; cytosolic thyroid hormone-binding protein; thyroid hormone-binding protein, cytosolic
• UniProt Protein Name: Pyruvate kinase PKM
• Protein Family: Pyruvate kinase
• UniProt Gene Name: PKM
• UniProt Synonym Gene Names: OIP3; PK2; PK3; PKM2; CTHBP; OIP-3; THBP1
• UniProt Entry Name: KPYM_HUMAN

NCBI Description

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]

Uniprot Description

PKM2: one of three rate-limiting enzymes in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule ATP and a pyruvate molecule, which is a central metabolic intermediate that can be used as a building block or oxidized further. Overexpressed in a variety of cancers. Interacts with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. Binds Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogens is. There are several mammalian isozymes of pyruvate kinase encoded by different genes. The L type predominates in liver, and the M type in muscle and brain. Two alternatively spliced human isoforms of PKM2 have been reported.

Protein type: Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Mitochondrial; EC 2.7.1.40; Nucleotide Metabolism - purine; Kinase, other

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: extracellular matrix; mitochondrion; cytoplasm; plasma membrane; cytosol; nucleus; vesicle; cilium

Molecular Function: potassium ion binding; protein binding; magnesium ion binding; pyruvate kinase activity; ATP binding

Biological Process: glycolysis; carbohydrate metabolic process; programmed cell death; glucose metabolic process; pathogenesis; phosphorylation