Rabbit anti-Human, Mouse PKLR Polyclonal Antibody | anti-PKLR antibody

PKLR, NT (Pyruvate Kinase Isozymes R/L, Pyruvate Kinase 1, R-type/L-type Pyruvate Kinase, Red Cell/Liver Pyruvate Kinase, PK1, PKL) (APC)

Cat. Num. AAA6496254

• Gene Names: PKLR; PK1; PKL; PKR; RPK; PKRL
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PKLR; Polyclonal Antibody; NT (Pyruvate Kinase Isozymes R/L; Pyruvate Kinase 1; R-type/L-type Pyruvate Kinase; Red Cell/Liver Pyruvate Kinase; PK1; PKL) (APC); anti-PKLR antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PKLR. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PKLR antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human PKLR (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-PKLR Pab is used in Western blot to detect PKLR in NIH/3T3 cell lysate.)

Western Blot (WB)

(PKLR Antibody (S8) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the PKLR antibody detected the PKLR protein (arrow).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-PKLR antibody

Antibodies; Enzymes

References

1. Wang, H., et al., Diabetes 51(9):2861-2865 (2002). 2. Valentini, G., et al., J. Biol. Chem. 277(26):23807-23814 (2002). 3. Mateu, E., et al., Hum. Genet. 110(6):532-544 (2002). 4. Indo, Y., et al., Hum. Mutat. 18(4):308-318 (2001). 5. Acan, N.L., et al., J. Enzym. Inhib. 16(5):457-464 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 10835121
• NCBI GeneID: 5313
• NCBI Accession #: NP_000289
• NCBI GenBank Nucleotide #: NM_000298.5
• UniProt Accession #: P30613; O75758; P11973
• Molecular Weight: 58,494 Da
• NCBI Official Full Name: pyruvate kinase PKLR isoform 1
• NCBI Official Synonym Full Names: pyruvate kinase L/R
• NCBI Official Symbol: PKLR
• NCBI Official Synonym Symbols: PK1; PKL; PKR; RPK; PKRL
• NCBI Protein Information: pyruvate kinase PKLR
• UniProt Protein Name: Pyruvate kinase PKLR
• Protein Family: Pyruvate kinase
• UniProt Gene Name: PKLR
• UniProt Synonym Gene Names: PK1; PKL
• UniProt Entry Name: KPYR_HUMAN

NCBI Description

The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PKLR: one of three rate-limiting enzymes in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule ATP and a pyruvate molecule, which is a central metabolic intermediate that can be used as a building block or oxidized further. There are several mammalian isozymes of pyruvate kinase encoded by different genes. The L type predominates in liver, and the M type in muscle and brain. Two alternatively spliced human isoforms have been described: the R- and L-types.

Protein type: Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other; Nucleotide Metabolism - purine; EC 2.7.1.40

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytosol

Molecular Function: potassium ion binding; magnesium ion binding; pyruvate kinase activity; ATP binding

Biological Process: pyruvate biosynthetic process; glycolysis; response to cAMP; response to lithium ion; positive regulation of cellular metabolic process; glucose metabolic process; pathogenesis; response to other organism; endocrine pancreas development; response to ATP; cellular response to insulin stimulus; response to heat; ATP biosynthetic process; carbohydrate metabolic process; response to hypoxia; energy reserve metabolic process; response to glucose stimulus; phosphorylation; response to nutrient

Disease: Adenosine Triphosphate, Elevated, Of Erythrocytes; Pyruvate Kinase Deficiency Of Red Cells