Rabbit anti-Human PKCtheta Polyclonal Antibody | anti-PKCtheta antibody

PKCtheta (Ab-676) Antibody

Cat. Num. AAA9412282

• Gene Names: PRKCQ; PRKCT; nPKC-theta
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• Synonyms: PKCtheta; Polyclonal Antibody; PKCtheta (Ab-676) Antibody; nPKC-theta; anti-PKCtheta antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total PKCth protein.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 643

• Applicable Applications for anti-PKCtheta antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500~1:1000; Immunohistochemistry: 1:50~1:100; Immunofluorescence: 1:100~1:200
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around aa. 674~678 (R-L-S-F-A) derived from Human PKCth.
• Target Name: PKCth
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Western Blot (WB)

(Western blot analysis of extracts from PC12 cells using PKCth(Ab-676) Antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCth(Ab-676) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed Hela cells using PKCth(Ab-676) Antibody.)

Related Product Information for anti-PKCtheta antibody

This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol. which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Product Categories/Family for anti-PKCtheta antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 334848126
• NCBI GeneID: 5588
• NCBI Accession #: NP_001229342.1
• NCBI GenBank Nucleotide #: NM_001242413.2
• UniProt Accession #: Q04759; Q14DH6; Q3MJF1; Q64FY5; Q9H508; Q9H549; B4DF52
• Molecular Weight: 67,560 Da
• NCBI Official Full Name: protein kinase C theta type isoform 2
• NCBI Official Synonym Full Names: protein kinase C, theta
• NCBI Official Symbol: PRKCQ
• NCBI Official Synonym Symbols: PRKCT; nPKC-theta
• NCBI Protein Information: protein kinase C theta type
• UniProt Protein Name: Protein kinase C theta type
• UniProt Gene Name: PRKCQ
• UniProt Synonym Gene Names: PRKCT
• UniProt Entry Name: KPCT_HUMAN

NCBI Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq, Jul 2008]

Uniprot Description

PKCT: an AGC kinase of the PKC family. A novel PKC: Ca2+ independent but still regulated by PS, DAG and phorbol esters. Contains 2 zinc-dependent phorbol-ester and DAG binding domains. Expressed in skeletal muscle and hematopoietic cells. May play a role in T cell receptor signaling and insulin resistance. Required for TCR-induced NF-kappaB activation in mature T lymphocytes.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; EC 2.7.11.13; Kinase, protein; AGC group; PKC family; Delta subfamily

Chromosomal Location of Human Ortholog: 10p15

Cellular Component: plasma membrane; immunological synapse; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; protein kinase C activity; metal ion binding; ubiquitin-protein ligase activity; ATP binding

Biological Process: positive regulation of interleukin-17 production; axon guidance; phototransduction, visible light; platelet activation; apoptosis; positive regulation of interleukin-2 biosynthetic process; membrane protein ectodomain proteolysis; protein ubiquitination; negative regulation of insulin receptor signaling pathway; T cell receptor signaling pathway; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; rhodopsin mediated signaling; positive regulation of interleukin-4 production; regulation of rhodopsin mediated signaling; regulation of transcription, DNA-dependent; innate immune response; positive regulation of T cell proliferation; regulation of cell growth; positive regulation of T cell activation; blood coagulation; inflammatory response; cell structure disassembly during apoptosis