Rabbit PKC zeta Polyclonal Antibody | anti-PRKCZ antibody

PKC zeta Antibody

Cat. Num. AAA9630892

• Gene Names: PRKCZ; PKC2; PKC-ZETA
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: PKC zeta; Polyclonal Antibody; PKC zeta Antibody; 14-3-3-zetaisoform; AI098070; aPKCzeta; C80388; EC 2.7.11.13; KPCZ_HUMAN; nPKC zeta; nPKC-zeta; OTTHUMP00000001368; OTTHUMP00000044160; PKC 2; PKC ZETA; PKC2; Pkcz; PKCZETA; PKM-zeta; included; PRKCZ; Protein kinase C zeta; Protein kinase C zeta form; Protein kinase C zeta type; r14-3-3; R74924; zetaPKC; anti-PRKCZ antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-PKC zeta (Thr410) Antibody detects endogenous levels of Phospho-PKC zeta (Thr410).
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PRKCZ antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human Phospho-PKC zeta (Thr410).
• Conjugation: Unconjugated
• Similarity: The PB1 domain mediate mutually exclusive interactions with SQSTM1 and PARD6B. The C1 domain does not bind the diacylglycerol (DAG). Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
• Post Translational Modifications: CDH5 is required for its phosphorylation at Thr-410. Phosphorylated by protein kinase PDPK1; phosphorylation is inhibited by the apoptotic C-terminal cleavage product of PKN2. Phosphorylation at Thr-410 by PI3K activates the kinase.
• Subunit Structure: Forms a ternary complex with SQSTM1 and KCNAB2. Forms another ternary complex with SQSTM1 and GABRR3. Forms a complex with SQSTM1 and MAP2K5 (By similarity). Interacts with PARD6A, PARD6B, PARD6G and SQSTM1. Part of a complex with PARD3, PARD6A or PARD6B or PARD6G and CDC42 or RAC1. Interacts with ADAP1/CENTA1. Forms a ternary complex composed of SQSTM1 and PAWR. Interacts directly with SQSTM1 (Probable). Interacts with IKBKB. Interacts (via the protein kinase domain) with WWC1. Forms a tripartite complex with WWC1 and DDR1, but predominantly in the absence of collagen. Component of the Par polarity complex, composed of at least phosphorylated PRKCZ, PARD3 and TIAM1. Interacts with PDPK1 (via N-terminal region). Interacts with WDFY2 (via WD repeats 1-3). Interacts with VAMP2. Forms a complex with WDFY2 and VAMP2. Interacts with APPL1.
• Tissue Specificity: Expressed in brain, and to a lesser extent in lung, kidney and testis.
• Function: Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation. Required for insulin-dependent activation of AKT3, but may function as an adapter rather than a direct activator. Upon insulin treatment may act as a downstream effector of PI3K and contribute to the activation of translocation of the glucose transporter SLC2A4/GLUT4 and subsequent glucose transport in adipocytes. In EGF-induced cells, binds and activates MAP2K5/MEK5-MAPK7/ERK5 independently of its kinase activity and can activate JUN promoter through MEF2C. Through binding with SQSTM1/p62, functions in interleukin-1 signaling and activation of NF-kappa-B with the specific adapters RIPK1 and TRAF6. Participates in TNF-dependent transactivation of NF-kappa-B by phosphorylating and activating IKBKB kinase, which in turn leads to the degradation of NF-kappa-B inhibitors. In migrating astrocytes, forms a cytoplasmic complex with PARD6A and is recruited by CDC42 to function in the establishment of cell polarity along with the microtubule motor and dynein. In association with FEZ1, stimulates neuronal differentiation in PC12 cells. In the inflammatory response, is required for the T-helper 2 (Th2) differentiation process, including interleukin production, efficient activation of JAK1 and the subsequent phosphorylation and nuclear translocation of STAT6. May be involved in development of allergic airway inflammation (asthma), a process dependent on Th2 immune response. In the NF-kappa-B-mediated inflammatory response, can relieve SETD6-dependent repression of NF-kappa-B target genes by phosphorylating the RELA subunit at 'Ser-311'. In vein endothelial cells treated with the oxidant peroxynitrite, phosphorylates STK11 leading to nuclear export of STK11, subsequent inhibition of PI3K/Akt signaling, and increased apoptosis. Phosphorylates VAMP2 in vitro.Involved in late synaptic long term potention phase in CA1 hippocampal cells and long term memory maintenance.
• Note: In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes (PubMed:9566925). Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction (PubMed:7597083). Colocalizes with VAMP2 and WDFY2 in intracellular vesicles (PubMed:17313651). Transiently translocates to the membrane of CA1 hippocampal cells in response to the induction of long term potentiation (By similarity).Cytoplasm.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-PRKCZ antibody

Cytoplasm; Endosome; Cell junction; Membrane; Peripheral membrane protein; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 75709226
• NCBI GeneID: 5590
• NCBI Accession #: NP_001028753.1
• NCBI GenBank Nucleotide #: NM_001033581.1
• UniProt Accession #: Q05513; Q15207; Q5SYT5; Q969S4; A8K4N0; A8MU64; B7Z2J7; E9PCW2
• Molecular Weight: 67,660 Da
• NCBI Official Full Name: protein kinase C zeta type isoform 2
• NCBI Official Synonym Full Names: protein kinase C, zeta
• NCBI Official Symbol: PRKCZ
• NCBI Official Synonym Symbols: PKC2; PKC-ZETA
• NCBI Protein Information: protein kinase C zeta type; nPKC-zeta
• UniProt Protein Name: Protein kinase C zeta type
• Protein Family: Protein kinase
• UniProt Gene Name: PRKCZ
• UniProt Synonym Gene Names: PKC2
• UniProt Entry Name: KPCZ_HUMAN

NCBI Description

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

PKCZ: an AGC kinase of the PKC family. An atypical PKC: its activity is not regulated by Ca2+, PS, DAG or phorbol esters. Constitutively active.

Protein type: EC 2.7.11.13; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; AGC group; PKC family; Iota subfamily

Chromosomal Location of Human Ortholog: 1p36.33-p36.2

Cellular Component: tight junction; nuclear matrix; apical cortex; leading edge; nuclear envelope; intercellular junction; cytosol; lipid raft; axon hillock; filamentous actin; membrane; perinuclear region of cytoplasm; apical plasma membrane; cytoplasm; plasma membrane; microtubule organizing center; cell junction; endosome

Molecular Function: protein serine/threonine kinase activity; protein domain specific binding; protein binding; protein kinase C activity; potassium channel regulator activity; phospholipase binding; insulin receptor substrate binding; metal ion binding; protein kinase binding; ATP binding; protein kinase activity

Biological Process: protein heterooligomerization; membrane hyperpolarization; negative regulation of insulin receptor signaling pathway; signal transduction; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; positive regulation of interleukin-4 production; positive regulation of glucose import; positive regulation of T-helper 2 cell differentiation; negative regulation of hydrolase activity; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; vesicle transport along microtubule; inflammatory response; positive regulation of cell-matrix adhesion; platelet activation; negative regulation of peptidyl-tyrosine phosphorylation; phospholipase D activation; cell migration; activation of protein kinase B; microtubule cytoskeleton organization and biogenesis; peptidyl-serine phosphorylation; long-term memory; establishment of cell polarity; actin cytoskeleton reorganization; insulin receptor signaling pathway; positive regulation of insulin receptor signaling pathway; blood coagulation; vascular endothelial growth factor receptor signaling pathway; negative regulation of protein complex assembly; negative regulation of apoptosis