Western Blot (WB)
(Western blot analysis of PKC iota/zeta expression in MCF7 (A), NIH3T3 (B), mouse kidney (C), rat kidney (D) whole cell lysates.)
Rabbit PKC iota/zeta Polyclonal Antibody | anti-PRKCI antibody
Anti-PKC iota/zeta Antibody
Western Blot (WB)
(Western blot analysis of PKC iota/zeta expression in MCF7 (A), NIH3T3 (B), mouse kidney (C), rat kidney (D) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of PKC iota/zeta expression in MCF7 (A), NIH3T3 (B), mouse kidney (C), rat kidney (D) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PKC iota/zeta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PKC iota/zeta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunofluorescence (IF)
(Immunofluorescent analysis of PKC iota/zeta staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of PKC iota/zeta staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X. [provided by RefSeq, Jul 2008]
Uniprot Description
PKCI: an AGC protein kinase of the PKC family. A calcium-independent, phospholipid-dependent atypical protein kinase C. Is not activated by phorbolesters or diaglycerol. May play role in the secretory response to nutrients. Involved in cell polarization processes and the formation of epithelial tight junctions. Predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets.
Protein type: Cell development/differentiation; Motility/polarity/chemotaxis; EC 2.7.11.13; Cell adhesion; Oncoprotein; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Tumor suppressor; Protein kinase, AGC; AGC group; PKC family; Iota subfamily
Chromosomal Location of Human Ortholog: 3q26.3
Cellular Component: apical plasma membrane; cytoplasm; cytosol; endosome; Golgi membrane; intercellular bridge; leading edge; microtubule cytoskeleton; nucleus; plasma membrane; protein complex; tight junction
Molecular Function: ATP binding; metal ion binding; phospholipid binding; protein binding; protein domain specific binding; protein kinase activity; protein kinase C activity; protein serine/threonine kinase activity
Biological Process: actin filament organization; activation of NF-kappaB transcription factor; cell migration; cellular response to insulin stimulus; cytoskeleton organization and biogenesis; establishment and/or maintenance of epithelial cell polarity; establishment of apical/basal cell polarity; eye photoreceptor cell development; Golgi vesicle budding; intercellular junction assembly and maintenance; negative regulation of apoptosis; negative regulation of neuron apoptosis; nerve growth factor receptor signaling pathway; peptidyl-serine phosphorylation; positive regulation of glucose import; protein amino acid phosphorylation; protein targeting to membrane; secretion; vesicle-mediated transport
Research Articles on PRKCI
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Product Notes
The PRKCI prkci (Catalog #AAA8242485) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PKC iota/zeta Antibody reacts with Human, Mouse, Rat, Dog, Rabbit, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's PKC iota/zeta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200); Immunofluorescence/Immunocytochemistry: (1/100 - 1/500). Researchers should empirically determine the suitability of the PRKCI prkci for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKC iota/zeta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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