Rabbit anti-Human, Mouse PKA alpha/beta CAT Polyclonal Antibody | anti-PKA alpha/beta CAT antibody

PKA alpha/beta CAT (Phospho-Thr197) Antibody

Cat. Num. AAA9408988

• Gene Names: PRKACA; PKACA; PPNAD4
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: PKA alpha/beta CAT; Polyclonal Antibody; PKA alpha/beta CAT (Phospho-Thr197) Antibody; PKA a/b CAT (Phospho-Thr197) Antibody; PRKACA; PRKACB; PKACA; anti-PKA alpha/beta CAT antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of PKA a/b CAT only when phosphorylated at threonine 197.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 427

• Applicable Applications for anti-PKA alpha/beta CAT antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500~1:1000; Immunohistochemistry: 1:50~1:100; Immunofluorescence: 1:100~1:200
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of threonine 197(T-W-T(p)-L-C) derived from Human PKA alpha/beta CAT.
• Modification: Phospho-Thr197
• Target Name: PKA a/b CAT
• Preparation and Storage: Store at -20 degree C/1 year

Western Blot (WB)

(Western blot analysis of extracts from mouse brain cells using PKA a/b CAT (Phospho-Thr197) Antibody.The lane on the right is treated with the antigen-specific peptide.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PKA a/b CAT (Phospho-Thr197) antibody (left)or the same antibody preincubated with blocking peptide (right).)

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed A549 cells using PKA a/b CAT (Phospho-Thr197) Antibody.)

Related Product Information for anti-PKA alpha/beta CAT antibody

Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts.

Product Categories/Family for anti-PKA alpha/beta CAT antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 748585205
• NCBI GeneID: 5566
• NCBI Accession #: NP_001291278.1
• NCBI GenBank Nucleotide #: NM_001304349.1
• UniProt Accession #: P17612; Q32P54; Q9H2Y0; Q9NRB4; Q9NRH9
• Molecular Weight: 39,822 Da
• NCBI Official Full Name: cAMP-dependent protein kinase catalytic subunit alpha isoform 3
• NCBI Official Synonym Full Names: protein kinase, cAMP-dependent, catalytic, alpha
• NCBI Official Symbol: PRKACA
• NCBI Official Synonym Symbols: PKACA; PPNAD4
• NCBI Protein Information: cAMP-dependent protein kinase catalytic subunit alpha
• UniProt Protein Name: cAMP-dependent protein kinase catalytic subunit alpha
• UniProt Gene Name: PRKACA
• UniProt Synonym Gene Names: PKACA; PKA C-alpha
• UniProt Entry Name: KAPCA_HUMAN

NCBI Description

This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. [provided by RefSeq, Jan 2015]

Uniprot Description

PKACA: catalytic subunit of cAMP-dependent protein kinase alpha, an AGC kinase. A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Two splice-variant isoforms have been described.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.11; Protein kinase, AGC; AGC group; PKA family

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: centrosome; mitochondrion; membrane; plasma membrane; nucleus; cytosol; neuromuscular junction; cAMP-dependent protein kinase complex; AMP-activated protein kinase complex

Molecular Function: protein binding; cAMP-dependent protein kinase activity; ubiquitin protein ligase binding; protein serine/threonine/tyrosine kinase activity; protein kinase binding; ATP binding

Biological Process: nerve growth factor receptor signaling pathway; water transport; protein amino acid autophosphorylation; pathogenesis; signal transduction; protein amino acid phosphorylation; positive regulation of protein export from nucleus; triacylglycerol catabolic process; G2/M transition of mitotic cell cycle; transmembrane transport; sperm capacitation; epidermal growth factor receptor signaling pathway; regulation of synaptic transmission; cytosolic calcium ion homeostasis; fibroblast growth factor receptor signaling pathway; regulation of osteoblast differentiation; regulation of heart rate; organelle organization and biogenesis; activation of protein kinase A; peptidyl-threonine phosphorylation; glucose metabolic process; gluconeogenesis; peptidyl-serine phosphorylation; mesoderm formation; phospholipase C activation; regulation of protein binding; carbohydrate metabolic process; neural tube closure; energy reserve metabolic process; innate immune response; renal water homeostasis; mitotic cell cycle; blood coagulation; regulation of insulin secretion