Western Blot (WB)
(Anti- PINX1 Picoband antibody, MBS178066, Western blottingAll lanes: Anti PINX1 (MBS178066) at 0.5ug/mlLane 1: Human Placenta Tissue Lysate at 50ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: HUT Whole Cell Lysate at 40ugLane 4: JURKAT Whole Cell Lysate at 40ugPredicted bind size: 37KDObserved bind size: 37KD )
anti-Human PINX1 Polyclonal Antibody | anti-PINX1 antibody
Anti-PINX1 Antibody
Gene Names
PINX1; LPTL; LPTS
Reactivity
Human
Applications
Western Blot
Purity
Immunogen Affinity Purified
Synonyms
PINX1; Polyclonal Antibody; Anti-PINX1 Antibody; PIN2/TERF1-interacting telomerase inhibitor 1; 67-11-3 protein antibodyFLJ20565; Hepatocellular carcinoma-related putative tumor suppressor; Liver-related putative tumor suppressor; LPTL; LPTS; MGC8850; OTTHUMP00000224984; OTTHUMP00000224985; OTTHUMP00000224986; OTTHUMP00000225053; OTTHUMP00000225054; PIN2 interacting protein 1; Pin2-interacting protein X1; PINX1_HUMAN; Protein 67-11-3; TRF1-interacting protein 1; PIN2/TERF1 interacting; telomerase inhibitor 1; anti-PINX1 antibody
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
174
Applicable Applications for anti-PINX1 antibody
Western Blot (WB)
Application Notes
Western Blot Concentration: 0.1-0.5ug/ml
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human PINX1 (54-83aa DHIKVQVKNNHLGLGATINNEDNWIAHQDD), different from the related mouse and rat sequences by three amino acids.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti- PINX1 Picoband antibody, MBS178066, Western blottingAll lanes: Anti PINX1 (MBS178066) at 0.5ug/mlLane 1: Human Placenta Tissue Lysate at 50ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: HUT Whole Cell Lysate at 40ugLane 4: JURKAT Whole Cell Lysate at 40ugPredicted bind size: 37KDObserved bind size: 37KD )
Western Blot (WB)
(Anti- PINX1 Picoband antibody, MBS178066, Western blottingAll lanes: Anti PINX1 (MBS178066) at 0.5ug/mlLane 1: Human Placenta Tissue Lysate at 50ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: HUT Whole Cell Lysate at 40ugLane 4: JURKAT Whole Cell Lysate at 40ugPredicted bind size: 37KDObserved bind size: 37KD )
Related Product Information for anti-PINX1 antibody
Description: Rabbit IgG polyclonal antibody for PIN2/TERF1-interacting telomerase inhibitor 1(PINX1) detection. Tested with WB in Human.
Background: PINX1, also known as PIN2 interacting protein 1, is a telomerase inhibitor and a possible tumor suppressor. It is mapped to 8p23. Over-expression of PINX1 results in decreased telomerase activity, telomere shortening, and induction of crisis. Reduction of PINX1 leads to an increase in telomerase activity and elongation of telomeres. PINX1 differs from other proteins that regulate telomere length in that it acts on telomerase while other proteins adjust telomere length without affecting telomerase activity. The PINX1 budding yeast orthologue Gnop1 inhibits telomerase by isolating the uncomplexed TERT protein so that it cannot associate with the telomerase template RNA, which prevents telomerase from being assembled. However, in humans, PINX1 impedes already assembled telomerase.
Background: PINX1, also known as PIN2 interacting protein 1, is a telomerase inhibitor and a possible tumor suppressor. It is mapped to 8p23. Over-expression of PINX1 results in decreased telomerase activity, telomere shortening, and induction of crisis. Reduction of PINX1 leads to an increase in telomerase activity and elongation of telomeres. PINX1 differs from other proteins that regulate telomere length in that it acts on telomerase while other proteins adjust telomere length without affecting telomerase activity. The PINX1 budding yeast orthologue Gnop1 inhibits telomerase by isolating the uncomplexed TERT protein so that it cannot associate with the telomerase template RNA, which prevents telomerase from being assembled. However, in humans, PINX1 impedes already assembled telomerase.
References
1. Banik, S.S.R.; Counter, C.M. (September 2004). "Characterization of Interactions between PinX1 and Human Telomerase Subunits hTERT and hTR". Journal of Biological Chemistry. 2. Zhou, X Z; Lu K P (Nov 2001). "The Pin2/TRF1-interacting protein PinX1 is a potent telomerase inhibitor". Cell (United States) 107 (3): 347-59.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,714 Da
NCBI Official Full Name
PIN2/TERF1-interacting telomerase inhibitor 1 isoform 2
NCBI Official Synonym Full Names
PIN2/TERF1 interacting, telomerase inhibitor 1
NCBI Official Symbol
PINX1
NCBI Official Synonym Symbols
LPTL; LPTS
NCBI Protein Information
PIN2/TERF1-interacting telomerase inhibitor 1
UniProt Protein Name
PIN2/TERF1-interacting telomerase inhibitor 1
Protein Family
UniProt Gene Name
PINX1
UniProt Synonym Gene Names
LPTL; LPTS
UniProt Entry Name
PINX1_HUMAN
Uniprot Description
PINX1: Microtubule-binding protein essential for faithful chromosome segregation. Mediates TRF1 and TERT accumulation in nucleolus and enhances TRF1 binding to telomeres. Inhibits telomerase activity. May inhibit cell proliferation and act as tumor suppressor. Belongs to the PINX1 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Inhibitor; Tumor suppressor; Nucleolus
Chromosomal Location of Human Ortholog: 8p23
Cellular Component: cytoplasm; intracellular membrane-bound organelle; kinetochore; mitochondrion; nuclear chromosome; nuclear chromosome, telomeric region; nucleolus; nucleus; spindle
Molecular Function: protein binding
Biological Process: mitotic metaphase plate congression; negative regulation of cell proliferation; negative regulation of protein ubiquitination; negative regulation of telomerase activity; negative regulation of telomere maintenance via telomerase; regulation of protein stability; regulation of telomerase activity; telomere maintenance via telomerase
Research Articles on PINX1
1. PinX1 protein expression is decreased in colorectal cancer patients and predicts a poor prognosis.
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Product Notes
The PINX1 pinx1 (Catalog #AAA178066) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-PINX1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PINX1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot Concentration: 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the PINX1 pinx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PINX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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