Immunohistochemistry (IHC)
(Anti-PIN1 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Rabbit PIN1 Polyclonal Antibody | anti-PIN1 antibody
Rabbit Polyclonal to Human PIN1
Usage: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 18 kD in size corresponding to Pin1 by western blotting in the...
Immunohistochemistry (IHC)
(Anti-PIN1 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC)
(Anti-PIN1 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Western Blot (WB)
(Anti-Pin1 Antibody - Western Blot. Western blot of affinity purified anti-Pin1 antibody to detect endogenous Pin1 in HeLa whole cell lysates. The sample was run in duplicate. A band representing Pin1 is indicated by the arrowhead. Cell lysates were electrophoresed using a straight 15% polyacrylamide gel, followed by transfer to nitrocellulose. The membrane was probed with the primary antibody at a 1:700 dilution. A 1:5000 dilution of HRP Gt-a-Rabbit IgG was used with a 15 sec exposure time. Personal Communication, L. D'Agostino and A. Giordano, SHRO, Philadelphia, PA.)
Western Blot (WB)
(Anti-Pin1 Antibody - Western Blot. Western blot of affinity purified anti-Pin1 antibody to detect endogenous Pin1 in HeLa whole cell lysates. The sample was run in duplicate. A band representing Pin1 is indicated by the arrowhead. Cell lysates were electrophoresed using a straight 15% polyacrylamide gel, followed by transfer to nitrocellulose. The membrane was probed with the primary antibody at a 1:700 dilution. A 1:5000 dilution of HRP Gt-a-Rabbit IgG was used with a 15 sec exposure time. Personal Communication, L. D'Agostino and A. Giordano, SHRO, Philadelphia, PA.)
NCBI and Uniprot Product Information
NCBI Description
Peptidyl-prolyl cis/trans isomerases (PPIases) catalyze the cis/trans isomerization of peptidyl-prolyl peptide bonds. This gene encodes one of the PPIases, which specifically binds to phosphorylated ser/thr-pro motifs to catalytically regulate the post-phosphorylation conformation of its substrates. The conformational regulation catalyzed by this PPIase has a profound impact on key proteins involved in the regulation of cell growth, genotoxic and other stress responses, the immune response, induction and maintenance of pluripotency, germ cell development, neuronal differentiation, and survival. This enzyme also plays a key role in the pathogenesis of Alzheimer's disease and many cancers. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011]
Uniprot Description
PIN1: a member of the parvulin family of peptidyl-prolyl isomerases (PPIase), has been implicated in the G2-M transition of the cell cycle. Has two distinct functional domains: an N-terminal WW domain and a C-terminal PPlase domain. Pin1 interacts with a series of mitotic phosphoproteins, including Plk1, cdc25C and cdc27, catalyzing pSer-Pro or pThr/Pro cis/trans isomerizations. Displays a preference for an acidic residue n-terminal to the isomerized proline bond.
Protein type: Nuclear receptor co-regulator; EC 5.2.1.8; Isomerase
Chromosomal Location of Human Ortholog: 19p13
Cellular Component: nucleoplasm; cytoplasm; nuclear speck; midbody; nucleus
Molecular Function: protein binding; peptidyl-prolyl cis-trans isomerase activity; mitogen-activated protein kinase kinase binding; phosphothreonine binding; GTPase activating protein binding; phosphoserine binding
Biological Process: protein peptidyl-prolyl isomerization; cytokine and chemokine mediated signaling pathway; positive regulation of ubiquitin-protein ligase activity; innate immune response; regulation of mitosis; positive regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; cell cycle; negative regulation of interferon type I production; regulation of cytokinesis
Research Articles on PIN1
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Product Notes
The PIN1 pin1 (Catalog #AAA248793) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human PIN1 reacts with Bovine, Dog, Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's PIN1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA). ELISA (1:2500 - 1:10000), IHC-P (5 ug/ml), IP (1:100), WB (1:500 - 1:3000) Usage: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 18 kD in size corresponding to Pin1 by western blotting in the.. Researchers should empirically determine the suitability of the PIN1 pin1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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