Western Blot (WB)
(Western blot analysis of extracts of rat kidney, using PIM1 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Rabbit anti-Rat Pim-1 Polyclonal Antibody | anti-PIM1 antibody
Pim-1 Rabbit pab Antibody
Gene Names
PIM1; PIM
Reactivity
Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Affinity purification
Synonyms
Pim-1; Polyclonal Antibody; Pim-1 Rabbit pab Antibody; PIM1; Pim-1 ; PIM; Proto-oncogene serine/threonine-protein kinase pim-1;; anti-PIM1 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
Liquid
Sequence Length
404
Applicable Applications for anti-PIM1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
Western Blot (WB): 1/1000-2000
Immunohistochemistry (IHC): 1/50-100
Immunocytochemistry (ICC): 1/50-100
Immunohistochemistry (IHC): 1/50-100
Immunocytochemistry (ICC): 1/50-100
Immunogen
A synthetic -peptide of human Pim-1
Buffer
PBS with 0.1% Sodium Azide, 50% Glycerol
Preparation and Storage
Upon delivery aliquot and store at -20 degree C or -80 degree C.
Western Blot (WB)
(Western blot analysis of extracts of rat kidney, using PIM1 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of rat kidney, using PIM1 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Related Product Information for anti-PIM1 antibody
Rabbit polyclonal to Human Pim-1
Pim proteins (Pim-1, Pim-2 and Pim-3) are oncogene-encoded serine/threonine kinases (1). Pim-1, a serine/threonine kinase highly expressed in hematopoietic cells, plays a critical role in the transduction of mitogenic signals and is rapidly induced by a variety of growth factors and cytokines (1-4). Pim-1 cooperates with c-Myc in lymphoid cell transformation and protects cells from growth factor withdrawal and genotoxic stress-induced apoptosis (5,6). Pim-1 also enhances the transcriptional activity of c-Myb through direct phosphorylation within the c-Myb DNA binding domain as well as phosphorylation of the transcriptional coactivator p100 (7,8). Hypermutations of the Pim-1 gene are found in B-cell diffuse large cell lymphomas (9). Phosphorylation of Pim-1 at Tyr218 by Etk occurs following IL-6 stimulation and correlates with an increase in Pim-1 activity (10). Various Pim substrates have been identified; Bad is phosphorylated by both Pim-1 and Pim-2 at Ser112 and this phosphorylation reverses Bad-induced cell apoptosis (11,12). The corresponding pim-1 gene encodes a pair of proteins through use of different translation initiation sites. Both larger 44 kDa (Pim-1L) and smaller 33 kDa (Pim-1S) proteins are active kinases, but differ in stability (13).
Pim proteins (Pim-1, Pim-2 and Pim-3) are oncogene-encoded serine/threonine kinases (1). Pim-1, a serine/threonine kinase highly expressed in hematopoietic cells, plays a critical role in the transduction of mitogenic signals and is rapidly induced by a variety of growth factors and cytokines (1-4). Pim-1 cooperates with c-Myc in lymphoid cell transformation and protects cells from growth factor withdrawal and genotoxic stress-induced apoptosis (5,6). Pim-1 also enhances the transcriptional activity of c-Myb through direct phosphorylation within the c-Myb DNA binding domain as well as phosphorylation of the transcriptional coactivator p100 (7,8). Hypermutations of the Pim-1 gene are found in B-cell diffuse large cell lymphomas (9). Phosphorylation of Pim-1 at Tyr218 by Etk occurs following IL-6 stimulation and correlates with an increase in Pim-1 activity (10). Various Pim substrates have been identified; Bad is phosphorylated by both Pim-1 and Pim-2 at Ser112 and this phosphorylation reverses Bad-induced cell apoptosis (11,12). The corresponding pim-1 gene encodes a pair of proteins through use of different translation initiation sites. Both larger 44 kDa (Pim-1L) and smaller 33 kDa (Pim-1S) proteins are active kinases, but differ in stability (13).
References
1. Mikkers, H. et al. (2004) Mol Cell Biol 24, 6104-15. 2. Selten, G. et al. (1986) Cell 46, 603-11. 3. Meeker, T.C. et al. (1987) J Cell Biochem 35, 105-12. 4. Dautry, F. et al. (1988) J Biol Chem 263, 17615-20. 5. Möröy, T. et al. (1993) Proc Natl Acad Sci USA 90, 10734-8. 6. Lilly, M. and Kraft, A. (1997) Cancer Res 57, 5348-55. 7. Leverson, J.D. et al. (1998) Mol Cell 2, 417-25. 8. Winn, L.M. et al. (2003) Cell Cycle 2, 258-62. 9. Pasqualucci, L. et al. (2001) Nature 412, 341-6. 10. Kim, O. et al. (2004) Oncogene 23, 1838-44. 11. Aho, T.L. et al. (2004) FEBS Lett 571, 43-9. 12. Yan, B. et al. (2003) J Biol Chem 278, 45358-67. 13. Saris, C.J. et al. (1991) EMBO J 10, 655-64.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
404
NCBI Official Full Name
serine/threonine-protein kinase pim-1 isoform 1
NCBI Official Synonym Full Names
pim-1 oncogene
NCBI Official Symbol
PIM1
NCBI Official Synonym Symbols
PIM
NCBI Protein Information
serine/threonine-protein kinase pim-1; Oncogene PIM1; pim-1 kinase 44 kDa isoform; pim-1 oncogene (proviral integration site 1); proto-oncogene serine/threonine-protein kinase pim-1
UniProt Protein Name
Serine/threonine-protein kinase pim-1
Protein Family
UniProt Gene Name
PIM1
UniProt Entry Name
PIM1_HUMAN
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Product Notes
The PIM1 pim1 (Catalog #AAA125957) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Pim-1 Rabbit pab Antibody reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Pim-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Western Blot (WB): 1/1000-2000 Immunohistochemistry (IHC): 1/50-100 Immunocytochemistry (ICC): 1/50-100. Researchers should empirically determine the suitability of the PIM1 pim1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pim-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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