Rabbit anti-Human, Mouse PIK3R3 Polyclonal Antibody | anti-PIK3R3 antibody

PIK3R3, CT (PIK3R3, Phosphatidylinositol 3-kinase regulatory subunit gamma, Phosphatidylinositol 3-kinase 55kD regulatory subunit gamma, p55PIK) (FITC)

Cat. Num. AAA6334083

• Gene Names: PIK3R3; p55; p55PIK; p55-GAMMA
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PIK3R3; Polyclonal Antibody; CT (PIK3R3; Phosphatidylinositol 3-kinase regulatory subunit gamma; Phosphatidylinositol 3-kinase 55kD regulatory subunit gamma; p55PIK) (FITC); p55PIK; anti-PIK3R3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PIK3R3 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PIK3R3 Antibody antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 324~353 amino acids from the C-terminal region of human PIK3R3.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The PIK3R3 polyclonal antibody is used in Western blot to detect PIK3R3 in mouse brain tissue lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PIK3R3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-PIK3R3 antibody

PIK3R3 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1. It is a component of a heterodimer of p110 (catalytic) and p55 (regulatory) subunits. The protein is expressed at highest levels in brain and testis. Lower levels are detected in adipose tissue, kidney, heart, lung and skeletal muscle. The protein contains 2 SH2 domains.

Product Categories/Family for anti-PIK3R3 antibody

Antibodies; Protein Kinases

References

Dey, B.R., et al., Gene 209 (1-2), 175-183 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 317373310
• NCBI GeneID: 8503
• UniProt Accession #: Q92569; O60482; Q5T4P1; Q5T4P2; B2R9C1; D3DQ12
• Molecular Weight: 461
• NCBI Official Full Name: Phosphatidylinositol 3-kinase regulatory subunit gamma
• NCBI Official Synonym Full Names: phosphoinositide-3-kinase, regulatory subunit 3 (gamma)
• NCBI Official Symbol: PIK3R3
• NCBI Official Synonym Symbols: p55; p55PIK; p55-GAMMA
• NCBI Protein Information: phosphatidylinositol 3-kinase regulatory subunit gamma; PI3-kinase subunit p55-gamma; PI3K regulatory subunit gamma; PI3-kinase regulatory subunit gamma; ptdIns-3-kinase regulatory subunit p55-gamma; phosphatidylinositol 3-kinase 55 kDa regulatory subunit
• UniProt Protein Name: Phosphatidylinositol 3-kinase regulatory subunit gamma
• Protein Family: Phosphatidylinositol 3-kinase regulatory
• UniProt Gene Name: PIK3R3
• UniProt Synonym Gene Names: PI3-kinase regulatory subunit gamma; PI3K regulatory subunit gamma; PtdIns-3-kinase regulatory subunit gamma; PI3-kinase subunit p55-gamma; PtdIns-3-kinase regulatory subunit p55-gamma
• UniProt Entry Name: P55G_HUMAN

Uniprot Description

PIK3R3: Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1. Heterodimer of a regulatory subunit PIK3R3 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with AXL. Highest levels in brain and testis. Lower levels in adipose tissue, kidney, heart, lung and skeletal muscle. Belongs to the PI3K p85 subunit family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Kinase, lipid

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: cytosol; phosphoinositide 3-kinase complex

Molecular Function: protein binding; 1-phosphatidylinositol-3-kinase activity; phosphoinositide 3-kinase regulator activity

Biological Process: phospholipid metabolic process; phosphatidylinositol biosynthetic process; insulin receptor signaling pathway; regulation of phosphoinositide 3-kinase activity