Rabbit anti-Human PIK3R2 Polyclonal Antibody | anti-PIK3R2 antibody

PIK3R2, NT (PIK3R2, Phosphatidylinositol 3-kinase regulatory subunit beta, Phosphatidylinositol 3-kinase 85kD regulatory subunit beta)

Cat. Num. AAA647186

• Gene Names: PIK3R2; p85; MPPH; P85B; p85-BETA
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: PIK3R2; Polyclonal Antibody; NT (PIK3R2; Phosphatidylinositol 3-kinase regulatory subunit beta; Phosphatidylinositol 3-kinase 85kD regulatory subunit beta); Anti -PIK3R2; anti-PIK3R2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-PIK3R2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100
• Immunogen: PIK3R2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5~35 amino acids from the N-terminal region of human PIK3R2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PIK3R2 Antibody (N-term) polyclonal antibody (arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIK3R2 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with PIK3R2 Antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-PIK3R2 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. PIK3R2 binds to activated Protein Tyrosine Kinases, which are phosphorylated, through its SH2 domain, and acts as an adaptor, mediating the association of the P110 catalytic unit to the plasma membrane.

Product Categories/Family for anti-PIK3R2 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 4826908
• NCBI GeneID: 5296
• NCBI Accession #: NP_005018.1
• NCBI GenBank Nucleotide #: NM_005027.3
• UniProt Accession #: O00459; Q5EAT5; Q9UPH9
• Molecular Weight: 81,545 Da
• NCBI Official Full Name: phosphatidylinositol 3-kinase regulatory subunit beta
• NCBI Official Synonym Full Names: phosphoinositide-3-kinase, regulatory subunit 2 (beta)
• NCBI Official Symbol: PIK3R2
• NCBI Official Synonym Symbols: p85; MPPH; P85B; p85-BETA
• NCBI Protein Information: phosphatidylinositol 3-kinase regulatory subunit beta; PI3-kinase subunit p85-beta; PI3K regulatory subunit beta; ptdIns-3-kinase regulatory subunit p85-beta; phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta)
• UniProt Protein Name: Phosphatidylinositol 3-kinase regulatory subunit beta
• Protein Family: Phosphatidylinositol 3-kinase regulatory
• UniProt Gene Name: PIK3R2
• UniProt Synonym Gene Names: PI3-kinase regulatory subunit beta; PI3K regulatory subunit beta; PtdIns-3-kinase regulatory subunit beta; PI3-kinase subunit p85-beta
• UniProt Entry Name: P85B_HUMAN

NCBI Description

Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates phosphatidylinositol and similar compounds, creating second messengers important in growth signaling pathways. PI3K functions as a heterodimer of a regulatory and a catalytic subunit. The protein encoded by this gene is a regulatory component of PI3K. Two transcript variants, one protein coding and the other non-protein coding, have been found for this gene. [provided by RefSeq, Dec 2012]

Uniprot Description

Function: Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.

Subunit structure: Heterodimer of a regulatory subunit PIK3R2 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with AXL. Interacts with FLT1 (tyrosine-phosphorylated) and FLT4 (tyrosine-phosphorylated). Interacts with NYAP1, NYAP2 and MYO16. Ref.5 Ref.6 Ref.7

Post-translational modification: Phosphorylated in response to signaling from activated receptor-type protein kinases. Dephosphorylated by PTPRJ. Ref.8

Involvement in disease: Megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome (MPPH) [MIM:603387]: A syndrome characterized by megalencephaly, hydrocephalus, and polymicrogyria; polydactyly may also be seen. There is considerable phenotypic similarity between this disorder and the megalencephaly-capillary malformation syndrome.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.14

Sequence similarities: Belongs to the PI3K p85 subunit family.Contains 1 Rho-GAP domain.Contains 2 SH2 domains.Contains 1 SH3 domain.