Rabbit anti-Human PIK3CD Polyclonal Antibody | anti-PIK3CD antibody

PIK3CD, Phosphorylated (Tyr485) (Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit delta Isoform, PtdIns-3-kinase Subunit delta, PI3-kinase Subunit delta, PI3K-delta, Phosphatidylinositol-4,5-bisphosphate 3-kinase 110kD Catalytic Subunit de

Cat. Num. AAA6493246

• Gene Names: PIK3CD; APDS; PI3K; IMD14; p110D; P110DELTA
• Reactivity: Human
• Applications: Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PIK3CD; Polyclonal Antibody; Phosphorylated (Tyr485) (Phosphatidylinositol-4; 5-bisphosphate 3-kinase Catalytic Subunit delta Isoform; PtdIns-3-kinase Subunit delta; PI3-kinase Subunit delta; PI3K-delta; Phosphatidylinositol-4; 5-bisphosphate 3-kinase 110kD Catalytic Subunit de; anti-PIK3CD antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PIK3CD when phosphorylated at Tyr485.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PIK3CD antibody: FLISA, Dot Blot (DB)
• Application Notes: FLISA: 1:1,000; Dot Blot: 1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Tyr485 of human PIK3CD (KLH).
• Conjugate: FITC
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-PIK3CD-pY485 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Product Categories/Family for anti-PIK3CD antibody

Antibodies; Protein Kinases

References

1. Khan, N.A., et al., J. Neurovirol. 9(6):584-593 (2003). 2. Sadhu, C., et al., J. Immunol. 170(5):2647-2654 (2003). 3. Deregibus, M.C., et al., J. Biol. Chem. 277(28):25195-25202 (2002). 4. Cook, J.A., et al., J. Immunol. 169(1):254-260 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 156564405
• NCBI GeneID: 5293
• NCBI Accession #: NP_005017
• NCBI GenBank Nucleotide #: NM_005026.3
• UniProt Accession #: O00329; O15445; Q5SR49; A6NCG0; G1FFP1
• Molecular Weight: 33,107 Da
• NCBI Official Full Name: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform
• NCBI Official Synonym Full Names: phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit delta
• NCBI Official Symbol: PIK3CD
• NCBI Official Synonym Symbols: APDS; PI3K; IMD14; p110D; P110DELTA
• NCBI Protein Information: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform; PI3-kinase p110 subunit delta; PI3Kdelta; phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta; phosphatidylinositol-4,5-bisphosphate 3-kinase cataly
• UniProt Protein Name: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform
• Protein Family: Phosphatidylinositol 4,5-bisphosphate 3-kinase
• UniProt Gene Name: PIK3CD
• UniProt Synonym Gene Names: PI3-kinase subunit delta; PI3K-delta; PI3Kdelta; PtdIns-3-kinase subunit delta; PtdIns-3-kinase subunit p110-delta; p110delta
• UniProt Entry Name: PK3CD_HUMAN

NCBI Description

Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I PI3K found primarily in leukocytes. Like other class I PI3Ks (p110-alpha p110-beta, and p110-gamma), the encoded protein binds p85 adapter proteins and GTP-bound RAS. However, unlike the other class I PI3Ks, this protein phosphorylates itself, not p85 protein.[provided by RefSeq, Jul 2010]

Uniprot Description

PIK3CD: Phosphoinositide-3-kinase (PI3K) that phosphorylates PftdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Mediates immune responses. Plays a role in B-cell development, proliferation, migration, and function. Required for B-cell receptor (BCR) signaling. Mediates B-cell proliferation response to anti-IgM, anti-CD40 and IL4 stimulation. Promotes cytokine production in response to TLR4 and TLR9. Required for antibody class switch mediated by TLR9. Involved in the antigen presentation function of B-cells. Involved in B-cell chemotaxis in response to CXCL13 and sphingosine 1-phosphate (S1P). Required for proliferation, signaling and cytokine production of naive, effector and memory T-cells. Required for T-cell receptor (TCR) signaling. Mediates TCR signaling events at the immune synapse. Activation by TCR leads to antigen-dependent memory T-cell migration and retention to antigenic tissues. Together with PIK3CG participates in T-cell development. Contributes to T-helper cell expansion and differentiation. Required for T-cell migration mediated by homing receptors SELL/CD62L, CCR7 and S1PR1 and antigen dependent recruitment of T-cells. Together with PIK3CG is involved in natural killer (NK) cell development and migration towards the sites of inflammation. Participates in NK cell receptor activation. Have a role in NK cell maturation and cytokine production. Together with PIK3CG is involved in neutrophil chemotaxis and extravasation. Together with PIK3CG participates in neutrophil respiratory burst. Have important roles in mast-cell development and mast cell mediated allergic response. Involved in stem cell factor (SCF)-mediated proliferation, adhesion and migration. Required for allergen-IgE-induced degranulation and cytokine release. The lipid kinase activity is required for its biological function. Isoform 2 may be involved in stabilizing total RAS levels, resulting in increased ERK phosphorylation and increased PI3K activity. Belongs to the PI3/PI4-kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - inositol phosphate; Kinase, lipid; EC 2.7.1.153; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: mast cell granule; plasma membrane; cytosol; phosphoinositide 3-kinase complex

Molecular Function: protein binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; 1-phosphatidylinositol-3-kinase activity; ATP binding; phosphoinositide 3-kinase activity; phosphatidylinositol-4-phosphate 3-kinase activity

Biological Process: respiratory burst during defense response; phosphoinositide 3-kinase cascade; T cell activation; nerve growth factor receptor signaling pathway; natural killer cell activation; signal transduction; T cell receptor signaling pathway; protein amino acid phosphorylation; B cell receptor signaling pathway; B cell homeostasis; phosphoinositide phosphorylation; natural killer cell differentiation; phospholipid metabolic process; inflammatory response; T cell differentiation; epidermal growth factor receptor signaling pathway; neutrophil chemotaxis; adaptive immune response; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; B cell activation; mast cell chemotaxis; cytokine production; mast cell degranulation; phosphatidylinositol biosynthetic process; innate immune response

Disease: Immunodeficiency 14