Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue)
Rabbit anti-Mouse Pigment Epithelium Derived Factor (PEDF) Polyclonal Antibody | anti-PEDF antibody
Polyclonal Antibody to Pigment Epithelium Derived Factor (PEDF)
Gene Names
Serpinf1; Pedf; Sdf3; EPC-1; Pedfl; AI195227
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Pigment Epithelium Derived Factor (PEDF); Polyclonal Antibody; Polyclonal Antibody to Pigment Epithelium Derived Factor (PEDF); anti-PEDF antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against PEDF. It has been selected for its ability to recognize PEDF in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- IAQLPLTGS MSIIFFLPLT VTQNLTMIEE SLTSEFIHDI DRELKTIQAV LTVPKLKLSF EGELTKSLQD MKLQSLFESP DFSKITGKPV KLTQVEHRAA FEWNEEGAGS SPSPGLQPVR LTFPLDYHLN QPFLFVLRDT DTGALLFIGR ILDPSST
MGHHHHHHSGSEF- IAQLPLTGS MSIIFFLPLT VTQNLTMIEE SLTSEFIHDI DRELKTIQAV LTVPKLKLSF EGELTKSLQD MKLQSLFESP DFSKITGKPV KLTQVEHRAA FEWNEEGAGS SPSPGLQPVR LTFPLDYHLN QPFLFVLRDT DTGALLFIGR ILDPSST
Sequence Length
418
Applicable Applications for anti-PEDF antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant PEDF (Ile262~Thr417) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2061306
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,234 Da
NCBI Official Full Name
pigment epithelium-derived factor
NCBI Official Synonym Full Names
serine (or cysteine) peptidase inhibitor, clade F, member 1
NCBI Official Symbol
Serpinf1
NCBI Official Synonym Symbols
Pedf; Sdf3; EPC-1; Pedfl; AI195227
NCBI Protein Information
pigment epithelium-derived factor
UniProt Protein Name
Pigment epithelium-derived factor
UniProt Gene Name
Serpinf1
UniProt Synonym Gene Names
Pedf; Sdf3; PEDF; SDF-3
Uniprot Description
PEDF: a secreted neurotrophic protein that induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. Extracellular phosphorylation enhances antiangiogenic activity.
Protein type: Inhibitor; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 11|11 B5
Cellular Component: axon; axon hillock; basement membrane; cell soma; extracellular exosome; extracellular matrix; extracellular region; extracellular space; perinuclear region of cytoplasm
Molecular Function: serine-type endopeptidase inhibitor activity
Biological Process: negative regulation of angiogenesis; negative regulation of endothelial cell migration; negative regulation of gene expression; negative regulation of inflammatory response; negative regulation of neuron death; positive regulation of neurogenesis; positive regulation of neuron projection development; short-term memory
Research Articles on PEDF
Similar Products
Product Notes
The PEDF serpinf1 (Catalog #AAA2006508) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Pigment Epithelium Derived Factor (PEDF) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Pigment Epithelium Derived Factor (PEDF) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the PEDF serpinf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- IAQLPLTGS MSIIFFLPLT VTQNLTMIEE SLTSEFIHDI DRELKTIQAV LTVPKLKLSF EGELTKSLQD MKLQSLFESP DFSKITGKPV KLTQVEHRAA FEWNEEGAGS SPSPGLQPVR LTFPLDYHLN QPFLFVLRDT DTGALLFIGR ILDPSST. It is sometimes possible for the material contained within the vial of "Pigment Epithelium Derived Factor (PEDF), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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