Rabbit PIAS3 Polyclonal Antibody | anti-PIAS3 antibody

Rabbit Polyclonal to Human PIAS3

Cat. Num. AAA243779

• Gene Names: PIAS3; ZMIZ5
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: PIAS3; Polyclonal Antibody; Rabbit Polyclonal to Human PIAS3; Anti-PIAS3 Antibody (C-Terminus) IHC-plus; E3 SUMO-protein ligase PIAS3; ZMIZ5; Human PIAS3; anti-PIAS3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Human PIAS3
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 628

• Applicable Applications for anti-PIAS3 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB (1 - 2 ug/ml); Usage: PIAS3 antibody can be used for detection of PIAS3 by Western blot at 1-2 ug/ml.
• Target Species: Human
• Immunogen Description: A 12 amino acid peptide near the carboxy terminus of human PIAS3.
• Immunogen Type: Synthetic peptide
• Immunogen: PIAS3 antibody was raised against a 12 amino acid peptide near the carboxy terminus of human PIAS3.
• Antigen Modification: C-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-PIAS3 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-PIAS3 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of PIAS3 in K562 cell lysate with PIAS3 antibody at 1 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 115298686
• NCBI GeneID: 10401
• NCBI Accession #: NP_006090.2
• NCBI GenBank Nucleotide #: NM_006099.3
• UniProt Accession #: Q9Y6X2; Q9UFI3
• Molecular Weight: 68,017 Da
• NCBI Official Full Name: E3 SUMO-protein ligase PIAS3
• NCBI Official Synonym Full Names: protein inhibitor of activated STAT, 3
• NCBI Official Symbol: PIAS3
• NCBI Official Synonym Symbols: ZMIZ5
• NCBI Protein Information: E3 SUMO-protein ligase PIAS3; zinc finger, MIZ-type containing 5; protein inhibitor of activated STAT protein 3
• UniProt Protein Name: E3 SUMO-protein ligase PIAS3
• Protein Family: E3 SUMO-protein ligase
• UniProt Gene Name: PIAS3
• UniProt Entry Name: PIAS3_HUMAN

NCBI Description

This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

PIAS3: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Monomer. Binds SUMO1 and UBE2I. Interacts with BCL11A, HMGA2, IRF1, MITF and NCOA2. Interacts with STAT5; the interaction occurs on stimulation by PRL. Interacts with GFI1; the interaction relieves the inhibitory effect of PIAS3 on STAT3- mediated transcriptional activity. Interacts with AR, PLAG1 and ZFHX3. Interacts with STAT3; the interaction occurs on stimulation by IL6, CNTF or OSM and inhibits the DNA binding activity of STAT3. By dihydrotestosterone (DHT) in prostate cancer cells. Isoform 1 is expressed in most tissues except thymus and small intestine. Isoform 3 is expressed only in brain, heart, thymus, muscle, lung, testis, lactating breast and embryonic stem cells. Belongs to the PIAS family.

Protein type: Nuclear receptor co-regulator; SUMO conjugating system; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytoplasm; dendrite; synapse; nucleus

Molecular Function: protein C-terminus binding; protein binding; potassium channel regulator activity; enzyme binding; zinc ion binding; protein N-terminus binding; SUMO ligase activity; ligase activity

Biological Process: positive regulation of protein sumoylation; protein sumoylation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; response to hormone stimulus; positive regulation of membrane potential