Rabbit PIAS3 Polyclonal Antibody | anti-PIAS3 antibody

PIAS3 Polyclonal Antibody

Cat. Num. AAA2561619

• Gene Names: PIAS3; ZMIZ5
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, ELISA
• Purity: Antigen Affinity Purification
• Synonyms: PIAS3; Polyclonal Antibody; PIAS3 Polyclonal Antibody; E3 SUMO protein ligase PIAS 3; E3 SUMO protein ligase PIAS3; E3 SUMO-protein ligase PIAS3; FLJ14651; OTTHUMP00000015586; OTTHUMP00000015587; PIAS 3; Pias3; PIAS3 protein; Protein inhibitor of activated STAT 3; Protein inhibitor of activated STAT protein 3; Protein inhibitor of activated STAT3; Zinc finger MIZ type containing 5; ZMIZ 5; ZMIZ5; anti-PIAS3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purification
• Form/Format: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
• Concentration: 0.6mg/mL (varies by lot)
• Sequence Length: 628

• Applicable Applications for anti-PIAS3 antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:25-1:100; ELISA: 1:5000-1:10000
• Immunogen: Synthetic peptide of human PIAS3
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human liver cancer tissue using PIAS3 Polyclonal Antibody at dilution of 1:30(×200))

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using PIAS3 Polyclonal Antibody at dilution of 1:30(×200))

Related Product Information for anti-PIAS3 antibody

This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined.

Product Categories/Family for anti-PIAS3 antibody

Cancer; Cell Biology; Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 115298686
• NCBI GeneID: 10401
• NCBI Accession #: NP_006090.2
• NCBI GenBank Nucleotide #: NP_006090.2
• UniProt Accession #: Q9Y6X2
• NCBI Official Full Name: E3 SUMO-protein ligase PIAS3
• NCBI Official Synonym Full Names: protein inhibitor of activated STAT 3
• NCBI Official Symbol: PIAS3
• NCBI Official Synonym Symbols: ZMIZ5
• NCBI Protein Information: E3 SUMO-protein ligase PIAS3
• UniProt Protein Name: E3 SUMO-protein ligase PIAS3
• Protein Family: E3 SUMO-protein ligase
• UniProt Gene Name: PIAS3
• UniProt Entry Name: PIAS3_HUMAN

NCBI Description

This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

PIAS3: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Monomer. Binds SUMO1 and UBE2I. Interacts with BCL11A, HMGA2, IRF1, MITF and NCOA2. Interacts with STAT5; the interaction occurs on stimulation by PRL. Interacts with GFI1; the interaction relieves the inhibitory effect of PIAS3 on STAT3- mediated transcriptional activity. Interacts with AR, PLAG1 and ZFHX3. Interacts with STAT3; the interaction occurs on stimulation by IL6, CNTF or OSM and inhibits the DNA binding activity of STAT3. By dihydrotestosterone (DHT) in prostate cancer cells. Isoform 1 is expressed in most tissues except thymus and small intestine. Isoform 3 is expressed only in brain, heart, thymus, muscle, lung, testis, lactating breast and embryonic stem cells. Belongs to the PIAS family.

Protein type: EC 6.3.2.-; SUMO conjugating system; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytoplasm; dendrite; synapse; nucleus

Molecular Function: protein C-terminus binding; protein binding; enzyme binding; potassium channel regulator activity; zinc ion binding; protein N-terminus binding; SUMO ligase activity; ligase activity

Biological Process: positive regulation of protein sumoylation; protein sumoylation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; response to hormone stimulus; positive regulation of membrane potential