Western Blot (WB)
(Western blot analysis of anti-PI3KR2 Pab in A549 cell lysate. PI3KR2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Rabbit anti-Human PI3KR2 Polyclonal Antibody | anti-PI3KR2 antibody
PI3KR2, CT (PIK3R2, Phosphatidylinositol 3-kinase regulatory subunit beta, Phosphatidylinositol 3-kinase 85kD regulatory subunit beta) (AP)
Gene Names
PIK3R2; p85; MPPH; P85B; p85-BETA
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
PI3KR2; Polyclonal Antibody; CT (PIK3R2; Phosphatidylinositol 3-kinase regulatory subunit beta; Phosphatidylinositol 3-kinase 85kD regulatory subunit beta) (AP); PIK3R2; Phosphatidylinositol 3-kinase 85kD regulatory subunit beta; anti-PI3KR2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-PI3KR2 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
PI3KR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276~305 amino acids from the C-terminal region of human PI3KR2.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis of anti-PI3KR2 Pab in A549 cell lysate. PI3KR2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Western Blot (WB)
(Western blot analysis of anti-PI3KR2 Pab in A549 cell lysate. PI3KR2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Related Product Information for anti-PI3KR2 antibody
PI3KR2 binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.
Product Categories/Family for anti-PI3KR2 antibody
References
Janssen, J.W., et al., Oncogene 16(13):1767-1772 (1998). Volinia, S., et al., Oncogene 7(4):789-793 (1992). Carpenter, C.L., et al., J. Biol. Chem. 265(32):19704-19711 (1990).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
728
NCBI Official Full Name
phosphatidylinositol 3-kinase regulatory subunit beta
NCBI Official Synonym Full Names
phosphoinositide-3-kinase, regulatory subunit 2 (beta)
NCBI Official Symbol
PIK3R2
NCBI Official Synonym Symbols
p85; MPPH; P85B; p85-BETA
NCBI Protein Information
phosphatidylinositol 3-kinase regulatory subunit beta; PI3-kinase subunit p85-beta; PI3K regulatory subunit beta; ptdIns-3-kinase regulatory subunit p85-beta; phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; phosphatidylinositol 3-kinase, reg
UniProt Protein Name
Phosphatidylinositol 3-kinase regulatory subunit beta
UniProt Gene Name
PIK3R2
UniProt Synonym Gene Names
PI3-kinase regulatory subunit beta; PI3K regulatory subunit beta; PtdIns-3-kinase regulatory subunit beta; PI3-kinase subunit p85-beta
UniProt Entry Name
P85B_HUMAN
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Product Notes
The PI3KR2 pik3r2 (Catalog #AAA6333728) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PI3KR2, CT (PIK3R2, Phosphatidylinositol 3-kinase regulatory subunit beta, Phosphatidylinositol 3-kinase 85kD regulatory subunit beta) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PI3KR2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-100 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PI3KR2 pik3r2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PI3KR2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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