Rabbit anti-Human PI3KC3 Polyclonal Antibody | anti-PI3KC3 antibody

PI3KC3, NT (PIK3C3, VPS34, Phosphatidylinositol 3-kinase catalytic subunit type 3, Phosphatidylinositol 3-kinase p100 subunit, Phosphoinositide-3-kinase class 3, hVps34) (Biotin)

Cat. Num. AAA6333642

• Gene Names: PIK3C3; VPS34; hVps34
• Reactivity: Human
• Applications: Immunofluorescence, ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PI3KC3; Polyclonal Antibody; NT (PIK3C3; VPS34; Phosphatidylinositol 3-kinase catalytic subunit type 3; Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide-3-kinase class 3; hVps34) (Biotin); PIK3C3; hVps34; anti-PI3KC3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PI3KC3 antibody: Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IF: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; IP: 1:500; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32~61 amino acids from the N-terminal region of human autophagy kinase PI3KC3.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-PI3KC3 Pab in HeLa cell lysate. PI3KC3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of PI3KC3 Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-PI3KC3 antibody

PI3KC3 is a catalytic subunit of the PI3K complex involved in the transport of lysosomal enzyme precursors to lysosomes. This enzyme acts catalytically to convert 1-phosphatidyl-1D-myo-inositol to 1-phosphatidyl-1D-myo-inositol 3-phosphate. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The regulation of the Beclin 1-PI3KC3 complex lipid kinase activity is a critical element in the autophagy signaling pathway.

Product Categories/Family for anti-PI3KC3 antibody

Antibodies; Protein Kinases

References

Vergne, I., et al., J. Exp. Med. 198(4):653-659 (2003). Volinia, S., et al., EMBO J. 14(14):3339-3348 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 34761064
• NCBI GeneID: 5289
• NCBI Accession #: NP_002638.2
• NCBI GenBank Nucleotide #: NM_002647.2
• UniProt Accession #: Q8NEB9; Q15134
• Molecular Weight: 887
• NCBI Official Full Name: phosphatidylinositol 3-kinase catalytic subunit type 3
• NCBI Official Synonym Full Names: phosphatidylinositol 3-kinase, catalytic subunit type 3
• NCBI Official Symbol: PIK3C3
• NCBI Official Synonym Symbols: VPS34; hVps34
• NCBI Protein Information: phosphatidylinositol 3-kinase catalytic subunit type 3; PI3K type 3; PI3-kinase type 3; PtdIns-3-kinase type 3; phosphoinositide-3-kinase, class 3; phosphatidylinositol 3-kinase p100 subunit
• UniProt Protein Name: Phosphatidylinositol 3-kinase catalytic subunit type 3
• UniProt Gene Name: PIK3C3
• UniProt Synonym Gene Names: VPS34; PI3-kinase type 3; PI3K type 3
• UniProt Entry Name: PK3C3_HUMAN