Rabbit anti-Human PHYH Polyclonal Antibody | anti-PHYH antibody

PHYH, NT (PHYH, PAHX, Phytanoyl-CoA dioxygenase, peroxisomal, Phytanic acid oxidase, Phytanoyl-CoA alpha-hydroxylase) (Azide free) (HRP)

Cat. Num. AAA6333501

• Gene Names: PHYH; RD; LN1; PAHX; LNAP1; PHYH1
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PHYH; Polyclonal Antibody; NT (PHYH; PAHX; Phytanoyl-CoA dioxygenase; peroxisomal; Phytanic acid oxidase; Phytanoyl-CoA alpha-hydroxylase) (Azide free) (HRP); Phytanoyl-CoA alpha-hydroxylase; anti-PHYH antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PHYH antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PHYH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66~95 amino acids from the N-terminal region of human PHYH.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PHYH Antibody (N-term) in MDA-MB435 cell line lysates (35ug/lane). PHYH (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PHYH Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of PHYH (arrow) using rabbit polyclonal PHYH Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PHYH gene.)

Flow Cytometry (FC/FACS)

(PHYH Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PHYH antibody

PHYH2 catalyzes a carbon-carbon cleavage reaction; cleaves a 2-hydroxy-3-methylacyl-CoA into formyl-CoA and a 2-methyl-branched fatty aldehyde.

Product Categories/Family for anti-PHYH antibody

Antibodies; Enzymes; Oxidase

References

Foulon, V., et.al., J. Biol. Chem. 280 (11), 9802-9812 (2005). Kikuchi, M., et.al., J. Biol. Chem. 279 (1), 421-428 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 83281447
• NCBI GeneID: 5264
• NCBI Accession #: NP_001032626.1
• NCBI GenBank Nucleotide #: NM_001037537.1
• UniProt Accession #: O14832; A8MTS8; B1ALH5
• Molecular Weight: 27,291 Da
• NCBI Official Full Name: phytanoyl-CoA dioxygenase, peroxisomal isoform b
• NCBI Official Synonym Full Names: phytanoyl-CoA 2-hydroxylase
• NCBI Official Symbol: PHYH
• NCBI Official Synonym Symbols: RD; LN1; PAHX; LNAP1; PHYH1
• NCBI Protein Information: phytanoyl-CoA dioxygenase, peroxisomal
• UniProt Protein Name: Phytanoyl-CoA dioxygenase, peroxisomal
• Protein Family: Phytanoyl-CoA dioxygenase
• UniProt Gene Name: PHYH
• UniProt Synonym Gene Names: PAHX; PhyH

NCBI Description

This gene is a member of the PhyH family and encodes a peroxisomal protein that is involved in the alpha-oxidation of 3-methyl branched fatty acids. Specifically, this protein converts phytanoyl-CoA to 2-hydroxyphytanoyl-CoA. Mutations in this gene have been associated with Refsum disease (RD) and deficient protein activity has been associated with Zellweger syndrome and rhizomelic chondrodysplasia punctata. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

Converts phytanoyl-CoA to 2-hydroxyphytanoyl-CoA.