Western Blot (WB)
(Western blot analysis of PHTNS Antibody (N-term) in Ramos cell line lysates (35ug/lane). PHTNS (arrow) was detected using the purified Pab.)
Rabbit anti-Human PHTNS Polyclonal Antibody | anti-PHTNS antibody
PHTNS, NT (PPP1R18, HKMT1098, KIAA1949, Phostensin, Protein phosphatase 1 F-actin cytoskeleton-targeting subunit, Protein phosphatase 1 regulatory subunit 18) (PE)
Gene Names
PPP1R18; HKMT1098; KIAA1949
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PHTNS; Polyclonal Antibody; NT (PPP1R18; HKMT1098; KIAA1949; Phostensin; Protein phosphatase 1 F-actin cytoskeleton-targeting subunit; Protein phosphatase 1 regulatory subunit 18) (PE); PPP1R18; Protein phosphatase 1 regulatory subunit 18; anti-PHTNS antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-PHTNS antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
PHTNS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 199~228 amino acids from the N-terminal region of human PHTNS.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis of PHTNS Antibody (N-term) in Ramos cell line lysates (35ug/lane). PHTNS (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of PHTNS Antibody (N-term) in Ramos cell line lysates (35ug/lane). PHTNS (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(PHTNS Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PHTNS Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(PHTNS Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PHTNS Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(PHTNS Antibody (N-term) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(PHTNS Antibody (N-term) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-PHTNS antibody
PHTNS interacts with regulatory subunits that target the enzyme to different cellular locations and change its activity toward specific substrates. Phostensin is a regulatory subunit that targets PP1 to F-actin cytoskeleton
Product Categories/Family for anti-PHTNS antibody
References
Lai, N.S., et al. Biochem. Biophys. Res. Commun. 387(4):676-681(2009). Barcellos, L.F., et al. PLoS Genet. 5 (10), E1000696 (2009). Kao, S.C., et al. Biochem. Biophys. Res. Commun. 356(3):594-598(2007).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17,771 Da
NCBI Official Full Name
phostensin
NCBI Official Synonym Full Names
protein phosphatase 1 regulatory subunit 18
NCBI Official Symbol
PPP1R18
NCBI Official Synonym Symbols
HKMT1098; KIAA1949
NCBI Protein Information
phostensin
UniProt Protein Name
Phostensin
UniProt Gene Name
PPP1R18
UniProt Synonym Gene Names
HKMT1098; KIAA1949
NCBI Description
Protein phosphatase-1 (PP1; see MIM 176875) interacts with regulatory subunits that target the enzyme to different cellular locations and change its activity toward specific substrates. Phostensin is a regulatory subunit that targets PP1 to F-actin (see MIM 102610) cytoskeleton (Kao et al., 2007 [PubMed 17374523]).[supplied by OMIM, Mar 2008]
Uniprot Description
PPP1R18: May target protein phosphatase 1 to F-actin cytoskeleton. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 6p21.33
Cellular Component: cytoplasm; cytoskeleton
Molecular Function: actin binding; phosphatase binding; protein binding
Research Articles on PHTNS
Similar Products
Product Notes
The PHTNS ppp1r18 (Catalog #AAA6333496) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PHTNS, NT (PPP1R18, HKMT1098, KIAA1949, Phostensin, Protein phosphatase 1 F-actin cytoskeleton-targeting subunit, Protein phosphatase 1 regulatory subunit 18) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PHTNS can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PHTNS ppp1r18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHTNS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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