Rabbit anti-Human Phosphorylase Kinase Subunit gamma 1 Polyclonal Antibody | anti-PHKG1 antibody

Phosphorylase Kinase Subunit gamma 1 (PHKG1, Phosphorylase B Kinase gamma Catalytic Chain Skeletal Muscle Isoform, PHKG) (Biotin)

Cat. Num. AAA6493324

• Gene Names: PHKG1; PHKG
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Phosphorylase Kinase Subunit gamma 1; Polyclonal Antibody; Phosphorylase Kinase Subunit gamma 1 (PHKG1; Phosphorylase B Kinase gamma Catalytic Chain Skeletal Muscle Isoform; PHKG) (Biotin); Phosphorylase Kinase; gamma-1 (Muscle); EC=2.7.11.19; anti-PHKG1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PHKG1.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PHKG1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human PHKG1 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-PHKG1 Pab in A375 cell lysate. PHKG1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-PHKG1 antibody

Antibodies; Protein Kinases

References

1. Wehner, M., et al., Hum. Genet. 96(5):616-618 (1995). 2. Jones, T.A., et al., Biochim. Biophys. Acta 1048(1):24-29 (1990).

NCBI and Uniprot Product Information

• NCBI GI #: 386642885
• NCBI GeneID: 5260
• NCBI Accession #: NP_001245388.1
• NCBI GenBank Nucleotide #: NM_001258459.1
• UniProt Accession #: Q16816; Q75LP5; B7Z1D0; F5H2S1
• Molecular Weight: 45,024 Da
• NCBI Official Full Name: phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform isoform 1
• NCBI Official Synonym Full Names: phosphorylase kinase, gamma 1 (muscle)
• NCBI Official Symbol: PHKG1
• NCBI Official Synonym Symbols: PHKG
• NCBI Protein Information: phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform; PHK-gamma-M; phosphorylase kinase gamma; phosphorylase kinase subunit gamma-1; serine/threonine-protein kinase PHKG1; phosphorylase b kinase gamma catalytic chain, skeletal muscl
• UniProt Protein Name: Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform
• Protein Family: Phosphorylase b kinase
• UniProt Gene Name: PHKG1
• UniProt Synonym Gene Names: PHKG; PHK-gamma-M
• UniProt Entry Name: PHKG1_HUMAN

NCBI Description

This gene is a member of the Ser/Thr protein kinase family and encodes a protein with one protein kinase domain and two calmodulin-binding domains. This protein is the catalytic member of a 16 subunit protein kinase complex which contains equimolar ratios of 4 subunit types. The complex is a crucial glycogenolytic regulatory enzyme. This gene has two pseudogenes at chromosome 7q11.21 and one at chromosome 11p11.12. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

Uniprot Description

PHKG1: a Ca(2+)/calmodulin-dependent protein kinase of the PHK family. Phosphorylase kinase is a polymer of 16 chains, four each of alpha, beta, gamma, and delta. Alpha and beta are regulatory chains, gamma is the catalytic chain, and delta is calmodulin.

Protein type: Kinase, protein; Protein kinase, CAMK; EC 2.7.11.26; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; EC 2.7.11.19; CAMK group; PHK family

Chromosomal Location of Human Ortholog: 7p11.2

Cellular Component: phosphorylase kinase complex; cytosol

Molecular Function: calmodulin binding; phosphorylase kinase activity; enzyme binding; tau-protein kinase activity; ATP binding

Biological Process: glycogen biosynthetic process; glycogen catabolic process; carbohydrate metabolic process; glucose metabolic process; pathogenesis; protein amino acid phosphorylation