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Western Blot (WB) (Western blot analysis of Phospholipase D4 expression in human tonsil (A), mouse testis (B), mouse muscle (C) whole cell lysates.)

Rabbit Phospholipase D4 Polyclonal Antibody | anti-PLD4 antibody

Anti-Phospholipase D4 Antibody

Gene Names
PLD4; C14orf175
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
Phospholipase D4; Polyclonal Antibody; Anti-Phospholipase D4 Antibody; C14orf175; PLD 4; Choline phosphatase 4; Phosphatidylcholine-hydrolyzing phospholipase D4; anti-PLD4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of Phospholipase D4 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
506
Applicable Applications for anti-PLD4 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB (1/500 - 1/1000)
IHC (1/100 - 1/200)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Phospholipase D4. The exact sequence is proprietary.
Preparation and Storage
Upon receipt aliquot and store at -20° C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of Phospholipase D4 expression in human tonsil (A), mouse testis (B), mouse muscle (C) whole cell lysates.)

Western Blot (WB) (Western blot analysis of Phospholipase D4 expression in human tonsil (A), mouse testis (B), mouse muscle (C) whole cell lysates.)

Western Blot (WB)

(Western blot analysis of Phospholipase D4 expression in PC12(A), CT26 (B), Hela (C), A549 (D) whole cell lysates.)

Western Blot (WB) (Western blot analysis of Phospholipase D4 expression in PC12(A), CT26 (B), Hela (C), A549 (D) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of Phospholipase D4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of Phospholipase D4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-PLD4 antibody
Rabbit polyclonal antibody to Phospholipase D4

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
phospholipase D4 isoform 2
NCBI Official Synonym Full Names
phospholipase D family, member 4
NCBI Official Symbol
PLD4
NCBI Official Synonym Symbols
C14orf175
NCBI Protein Information
phospholipase D4
UniProt Protein Name
Phospholipase D4
Protein Family
UniProt Gene Name
PLD4
UniProt Synonym Gene Names
C14orf175; PLD 4
UniProt Entry Name
PLD4_HUMAN

Uniprot Description

PLD4: Belongs to the phospholipase D family.

Protein type: Phospholipase; Membrane protein, integral; EC 3.1.4.4

Chromosomal Location of Human Ortholog: 14q32.33

Cellular Component: endoplasmic reticulum membrane; integral to membrane; trans-Golgi network membrane; phagocytic vesicle; nucleus

Molecular Function: phospholipase D activity; phosphoinositide phospholipase C activity

Biological Process: inositol phosphate metabolic process; phosphatidylglycerol biosynthetic process; phospholipid metabolic process; glycerophospholipid biosynthetic process; hemopoietic progenitor cell differentiation; lipid catabolic process; phagocytosis

Research Articles on PLD4

Similar Products

Product Notes

The PLD4 pld4 (Catalog #AAA8227575) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Phospholipase D4 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Phospholipase D4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB (1/500 - 1/1000) IHC (1/100 - 1/200). Researchers should empirically determine the suitability of the PLD4 pld4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospholipase D4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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