Western Blot (WB)
(Western Blot: Lane1: Human Hela cells; Lane2: Porcine Brain Tissue.)
Rabbit anti-Human Phosphoinositide-3-Kinase Class 3 Polyclonal Antibody | anti-PIK3C3 antibody
Polyclonal Antibody to Phosphoinositide-3-Kinase Class 3 (PIK3C3)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- YPVIFKHGDD LRQDQLILQI ISLMDKLLRK ENLDLKLTPY KVLATSTKHG FMQFIQSVPV AEVLDTEGSI QNFFRKYAPS ENGPNGISAE VMDTYVKSCA GYCVITYILG VGDRHLDNLL LTKTGKLFHI DFGYILGRDP KPLPPPMKLN KEMVEGMGGT QSEQYQEFRK QCYTAFLHLR RYSNLILNLF SLMVDANIPD IALEPDKTVK KVQDKFRLDL SDEEAVHYMQ SLIDESVHAL FAAVVEQIHK FAQYW
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Lane1: Human Hela cells; Lane2: Porcine Brain Tissue.)
Western Blot (WB)
(Western Blot: Lane1: Human Hela cells; Lane2: Porcine Brain Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant PIK3C3, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant PIK3C3, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Pancreas Cancer Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Pancreas Cancer Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Breast Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Breast Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue)
NCBI and Uniprot Product Information
Uniprot Description
PIK3C3: Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. Required for transport from early to late endosomes. Heterodimer. This subunit, part of a complex composed of regulatory and catalytic subunits, associates with regulatory subunit PIK3R4. Forms a complex with BECN1, PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. In this complex, presence of UVRAG and ATG14 are mutually exclusive. Part of a complex composed of PIK3R4 and PIK3CB. Interacts with RAB7A in the presence of PIK3R4. Ubiquitously expressed, with a highest expression in skeletal muscle. Belongs to the PI3/PI4-kinase family.
Protein type: Autophagy; Carbohydrate Metabolism - inositol phosphate; EC 2.7.1.137; Kinase, lipid; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 18q12.3
Cellular Component: autolysosome; axoneme; cytosol; late endosome; membrane; midbody; peroxisome; phagocytic vesicle membrane; phagophore assembly site
Molecular Function: 1-phosphatidylinositol-3-kinase activity; ATP binding; kinase activity; protein binding; protein kinase activity
Biological Process: autophagosome assembly; autophagy; autophagy of peroxisome; cellular response to glucose starvation; cytokinesis; early endosome to late endosome transport; endocytosis; endosome organization and biogenesis; interleukin-7-mediated signaling pathway; macroautophagy; phosphatidylinositol biosynthetic process; phosphatidylinositol phosphorylation; phosphoinositide-mediated signaling; protein amino acid phosphorylation; protein lipidation; protein localization to phagophore assembly site; protein processing; regulation of cytokinesis; regulation of protein secretion; response to leucine; toll-like receptor 9 signaling pathway
Research Articles on PIK3C3
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Product Notes
The PIK3C3 pik3c3 (Catalog #AAA2025873) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Phosphoinositide-3-Kinase Class 3 (PIK3C3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Phosphoinositide-3-Kinase Class 3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1-5ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the PIK3C3 pik3c3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- YPVIFKHGDD LRQDQLILQI ISLMDKLLRK ENLDLKLTPY KVLATSTKHG FMQFIQSVPV AEVLDTEGSI QNFFRKYAPS ENGPNGISAE VMDTYVKSCA GYCVITYILG VGDRHLDNLL LTKTGKLFHI DFGYILGRDP KPLPPPMKLN KEMVEGMGGT QSEQYQEFRK QCYTAFLHLR RYSNLILNLF SLMVDANIPD IALEPDKTVK KVQDKFRLDL SDEEAVHYMQ SLIDESVHAL FAAVVEQIHK FAQYW. It is sometimes possible for the material contained within the vial of "Phosphoinositide-3-Kinase Class 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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