Rabbit anti-Human Phosphoglycerate Kinase 1 Polyclonal Antibody | anti-PGK1 antibody

Phosphoglycerate Kinase 1 (PGK1, Cell Migration-inducing Gene 10 Protein, MGC8947, MGC117307, MGC142128, MIG10, OK/SW-cl.110, PGKA, Primer Recognition Protein 2, PRP 2) (FITC)

Cat. Num. AAA6493125

• Gene Names: PGK1; PGKA; MIG10; HEL-S-68p
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Phosphoglycerate Kinase 1; Polyclonal Antibody; Phosphoglycerate Kinase 1 (PGK1; Cell Migration-inducing Gene 10 Protein; MGC8947; MGC117307; MGC142128; MIG10; OK/SW-cl.110; PGKA; Primer Recognition Protein 2; PRP 2) (FITC); Migration-inducing Gene 10 Protein (Phosphoglycerate Kinase 1); EC=2.7.2.3; anti-PGK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PGK1.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PGK1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human PGK1 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PGK1 Antibody (Center S320), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of hPGK1-S320 in Hela, HepG2 cell line lysates (35ug/lane). PGK1 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of PGK1 (arrow) using rabbit polyclonal PGK1 Antibody (S320). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PGK1 gene.)

Product Categories/Family for anti-PGK1 antibody

Antibodies; Protein Kinases

References

1. Shetty, S., et al., Am. J. Respir. Cell Mol. Biol. 31(1):100-106 (2004). 2. Daly, E.B., et al., Biochim. Biophys. Acta 1691(1):17-22 (2004). 3. Daly, E.B., et al., Int. J. Biol. Markers 19(2):170-172 (2004). 4. Saito, Y., et al., Biochem. Biophys. Res. Commun. 314(2):396-402 (2004). 5. Krishnan, P., et al., J. Biol. Chem. 278(38):36726-36732 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4505763
• NCBI GeneID: 5230
• NCBI Accession #: NP_000282
• NCBI GenBank Nucleotide #: NM_000291.3
• UniProt Accession #: Q5J7W1; Q6IBT6; Q8NI87; A8K4W6; B7Z7A9; P00558
• Molecular Weight: 41,429 Da
• NCBI Official Full Name: phosphoglycerate kinase 1
• NCBI Official Synonym Full Names: phosphoglycerate kinase 1
• NCBI Official Symbol: PGK1
• NCBI Official Synonym Symbols: PGKA; MIG10; HEL-S-68p
• NCBI Protein Information: phosphoglycerate kinase 1
• Protein Family: Phosphoglycerate kinase

NCBI Description

The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. [provided by RefSeq, Jan 2014]