Rabbit anti-Human, Mouse Phosphofructokinase, Liver Polyclonal Antibody | anti-PFKL antibody

Phosphofructokinase, Liver, CT (PFKL, 6-phosphofructokinase Liver Type, DKFZp686G1648, DKFZp686L2097, FLJ30173, FLJ40909, Phosphofructo-1-kinase Isozyme B, PFK-B, Phosphofructokinase 1, Phosphohexokinase) (FITC)

Cat. Num. AAA6493103

• Gene Names: PFKL; PFK-B
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Phosphofructokinase; Liver; Polyclonal Antibody; CT (PFKL; 6-phosphofructokinase Liver Type; DKFZp686G1648; DKFZp686L2097; FLJ30173; FLJ40909; Phosphofructo-1-kinase Isozyme B; PFK-B; Phosphofructokinase 1; Phosphohexokinase) (FITC); EC=2.7.1.11; anti-PFKL antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PFKL. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PFKL antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human PFKL (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-PFKL Pab is used in Western blot to detect PFKL in HepG2 cell lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-PFKL antibody

Antibodies; Protein Kinases

References

1. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002). 2. Hattori, M., et al., Nature 405(6784):311-319 (2000). 3. Elson, A., et al., Genomics 7(1):47-56 (1990). 4. Levanon, D., et al., DNA 8(10):733-743 (1989).

NCBI and Uniprot Product Information

• NCBI GI #: 48762920
• NCBI GeneID: 5211
• NCBI Accession #: NP_002617.3
• NCBI GenBank Nucleotide #: NM_002626.4
• UniProt Accession #: P17858; Q96A64; Q96IH4; Q9BR91
• Molecular Weight: 85,018 Da
• NCBI Official Full Name: 6-phosphofructokinase, liver type
• NCBI Official Synonym Full Names: phosphofructokinase, liver
• NCBI Official Symbol: PFKL
• NCBI Official Synonym Symbols: PFK-B
• NCBI Protein Information: 6-phosphofructokinase, liver type; phosphohexokinase; phosphofructokinase 1; liver-type 1-phosphofructokinase; phosphofructo-1-kinase isozyme B
• UniProt Protein Name: 6-phosphofructokinase, liver type
• Protein Family: ATP-dependent 6-phosphofructokinase
• UniProt Gene Name: PFKL
• UniProt Synonym Gene Names: PFK-B
• UniProt Entry Name: K6PL_HUMAN

NCBI Description

Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. The protein encoded by this gene represents the L subunit. Alternate splicing results in two transcript variants, one of which is a candidate for nonsense-mediated decay (NMD). [provided by RefSeq, Aug 2008]

Uniprot Description

PFKL: phosphofructokinase, liver type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts. Two alternatively spliced isoforms have been described.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pentose phosphate pathway; EC 2.7.1.11; Carbohydrate Metabolism - galactose; Kinase, other; Carbohydrate Metabolism - fructose and mannose

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: 6-phosphofructokinase complex; membrane; cytosol

Molecular Function: identical protein binding; protein binding; metal ion binding; kinase binding; ATP binding; 6-phosphofructokinase activity

Biological Process: fructose 1,6-bisphosphate metabolic process; glycolysis; carbohydrate metabolic process; carbohydrate phosphorylation; response to glucose stimulus; glucose metabolic process; pathogenesis; fructose 6-phosphate metabolic process; protein oligomerization; protein homotetramerization; negative regulation of insulin secretion