Rabbit Phospho-ABL (Y412) Polyclonal Antibody | anti-ABL1 antibody

Phospho-ABL (Y412) Antibody

Cat. Num. AAA9204367

• Gene Names: ABL1; ABL; JTK7; p150; c-ABL; v-abl; c-ABL1; bcr/abl
• Reactivity: Human; (Predicted Reactivity: Zebrafish, Mouse)
• Applications: Western Blot, Dot Blot, ELISA, Immunohistochemistry
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: Phospho-ABL (Y412); Polyclonal Antibody; Phospho-ABL (Y412) Antibody; Tyrosine-protein kinase ABL1; Abelson murine leukemia viral oncogene homolog 1; Abelson tyrosine-protein kinase 1; Proto-oncogene c-Abl; p150; ABL1; ABL; JTK7; anti-ABL1 antibody

• Host: Rabbit
• Reactivity: Human; (Predicted Reactivity: Zebrafish, Mouse)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This ABL Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y393 of human ABL1 or Y439 of human ABL2.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Length: 1,149

• Applicable Applications for anti-ABL1 antibody: Western Blot (WB), Dot Blot (DB), ELISA (EIA), Immunohistochemistry Paraffin (IHC-P)
• Application Notes: WB~~1:1000; IHC-P~~1:50~100; 1:500
• Cellular Location: Cytoplasm, cytoskeleton. Nucleus. Mitochondrion. Note=Shuttles between the nucleus and cytoplasm depending on environmental signals. Sequestered into the cytoplasm through interaction with 14-3-3 proteins. Localizes to mitochondria in response to oxidative stress (By similarity).
• Tissue Location: Widely expressed.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of Phospho-ABL-Y412 Antibody Pab in A2058 cell line lysate (35ug/lane). ABL (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Dot Blot (DB)

(Dot blot analysis of Phospho-ABL1 (Y393)/ABL2 (439) Antibody Pab (MBS9204367) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Related Product Information for anti-ABL1 antibody

Function: Non-receptor tyrosine-protein kinase that plays a role in many key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion, receptor endocytosis, autophagy, DNA damage response and apoptosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like WASF3 (involved in branch formation); ANXA1 (involved in membrane anchoring); DBN1, DBNL, CTTN, RAPH1 and ENAH (involved in signaling); or MAPT and PXN (microtubule-binding proteins). Phosphorylation of WASF3 is critical for the stimulation of lamellipodia formation and cell migration. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as BCAR1, CRK, CRKL, DOK1, EFS or NEDD9. Phosphorylates multiple receptor tyrosine kinases and more particularly promotes endocytosis of EGFR, facilitates the formation of neuromuscular synapses through MUSK, inhibits PDGFRB-mediated chemotaxis and modulates the endocytosis of activated B-cell receptor complexes. Other substrates which are involved in endocytosis regulation are the caveolin (CAV1) and RIN1. Moreover, ABL1 regulates the CBL family of ubiquitin ligases that drive receptor down-regulation and actin remodeling. Phosphorylation of CBL leads to increased EGFR stability. Involved in late-stage autophagy by regulating positively the trafficking and function of lysosomal components. ABL1 targets to mitochondria in response to oxidative stress and thereby mediates mitochondrial dysfunction and cell death. In response to oxidative stress, phosphorylates serine/threonine kinase PRKD2 at 'Tyr-717' (PubMed:28428613). ABL1 is also translocated in the nucleus where it has DNA-binding activity and is involved in DNA-damage response and apoptosis. Many substrates are known mediators of DNA repair: DDB1, DDB2, ERCC3, ERCC6, RAD9A, RAD51, RAD52 or WRN. Activates the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates TP73, a primary regulator for this type of damage-induced apoptosis. Phosphorylates the caspase CASP9 on 'Tyr-153' and regulates its processing in the apoptotic response to DNA damage. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks. ABL1 acts also as a regulator of multiple pathological signaling cascades during infection. Several known tyrosine-phosphorylated microbial proteins have been identified as ABL1 substrates. This is the case of A36R of Vaccinia virus, Tir (translocated intimin receptor) of pathogenic E.coli and possibly Citrobacter, CagA (cytotoxin-associated gene A) of H.pylori, or AnkA (ankyrin repeat-containing protein A) of A.phagocytophilum. Pathogens can highjack ABL1 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1. Regulates T-cell differentiation in a TBX21-dependent manner. Phosphorylates TBX21 on tyrosine residues leading to an enhancement of its transcriptional activator activity (By similarity).

Product Categories/Family for anti-ABL1 antibody

Cancer; Signal Transduction; Phospho Antibodies

References

Cao, C., et al., Biochemistry 42(35):10348-10353 (2003). Cao, C., et al., J. Biol. Chem. 278(32):29667-29675 (2003). Kruh, G.D., et al., Proc. Natl. Acad. Sci. U.S.A. 87(15):5802-5806 (1990). Kruh, G.D., et al., Science 234(4783):1545-1548 (1986). Bianchi, C., et al., FEBS Lett. 527 (1-3), 216-222 (2002) (): ().

NCBI and Uniprot Product Information

• NCBI GI #: 62362414
• NCBI GeneID: 25
• NCBI Accession #: NP_005148.2; NP_009297.2
• NCBI GenBank Nucleotide #: NM_005157.5
• UniProt Accession #: P00519; Q13869; Q13870; Q16133; Q17R61; Q45F09; A3KFJ3
• NCBI Official Full Name: tyrosine-protein kinase ABL1 isoform a
• NCBI Official Synonym Full Names: ABL proto-oncogene 1, non-receptor tyrosine kinase
• NCBI Official Symbol: ABL1
• NCBI Official Synonym Symbols: ABL; JTK7; p150; c-ABL; v-abl; c-ABL1; bcr/abl
• NCBI Protein Information: tyrosine-protein kinase ABL1
• UniProt Protein Name: Tyrosine-protein kinase ABL1
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: ABL1
• UniProt Synonym Gene Names: ABL; JTK7
• UniProt Entry Name: ABL1_HUMAN

NCBI Description

This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons. [provided by RefSeq, Aug 2014]

Uniprot Description

Abl: an ubiquitously expressed and highly conserved proto-oncogenic tyrosine kinase. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. Negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. It has been implicated in regulation of cell proliferation, differentiation, apoptosis, cell adhesion and stress response. The Philadephia chromosome translocation t(9;22)(q34;q11) creates a Bcr-Abl fusion protein, responsible for 90% of chronic myelogenous leukemia (CML) and ~25% of acute lymphoblastic leukemia (ALL). Inhibitors: Gleevec (imatinib, Glivec), Dasatinib. Two alternatively-spliced isoforms have been described.

Protein type: Protein kinase, TK; Kinase, protein; Oncoprotein; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; TK group; Abl family

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: nucleoplasm; extrinsic to internal side of plasma membrane; nuclear membrane; mitochondrion; perinuclear region of cytoplasm; leading edge; cytoplasm; nucleolus; cytosol; nucleus; actin cytoskeleton

Molecular Function: actin monomer binding; protein C-terminus binding; non-membrane spanning protein tyrosine kinase activity; magnesium ion binding; protein kinase activity; actin filament binding; protein binding; DNA binding; syntaxin binding; protein-tyrosine kinase activity; manganese ion binding; mitogen-activated protein kinase binding; nicotinate-nucleotide adenylyltransferase activity; SH3 domain binding; ATP binding; receptor binding

Biological Process: DNA damage induced protein phosphorylation; positive regulation of apoptosis; cerebellum morphogenesis; negative regulation of BMP signaling pathway; B cell receptor signaling pathway; elevation of cytosolic calcium ion concentration; transitional one stage B cell differentiation; regulation of endocytosis; negative regulation of mitotic cell cycle; neuromuscular process controlling balance; cell cycle arrest; positive regulation of oxidoreductase activity; spleen development; mitosis; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of I-kappaB kinase/NF-kappaB cascade; muscle cell differentiation; positive regulation of peptidyl-tyrosine phosphorylation; DNA damage response, signal transduction resulting in induction of apoptosis; positive regulation of osteoblast proliferation; negative regulation of ubiquitin-protein ligase activity; autophagy; response to oxidative stress; actin cytoskeleton organization and biogenesis; axon guidance; peptidyl-tyrosine phosphorylation; alpha-beta T cell differentiation; platelet-derived growth factor receptor signaling pathway; mitochondrial depolarization; positive regulation of mitotic cell cycle; Bergmann glial cell differentiation; regulation of transcription, DNA-dependent; collateral sprouting; cell differentiation; B cell proliferation during immune response; epidermal growth factor receptor signaling pathway; regulation of cell adhesion; cell migration; mismatch repair; thymus development; DNA damage response, signal transduction; B-1 B cell homeostasis; activated T cell proliferation; protein modification process; regulation of cell proliferation; microspike biogenesis; positive regulation of muscle cell differentiation; innate immune response; regulation of autophagy; negative regulation of cell-cell adhesion; positive regulation of release of sequestered calcium ion into cytosol; blood coagulation; response to DNA damage stimulus

Disease: Leukemia, Chronic Myeloid