Rabbit PHOQ Polyclonal Antibody | anti-PHOQ antibody
Rabbit anti-Escherichia coli (strain K12) PHOQ Polyclonal Antibody
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH7.4
2. 1mL preimmune serum (negative control)
3. 10mg rabbit polyclonal antibody purified by Protein A/G.
2. Antibody titer > 1: 64,000 confirmed by ELISA
3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
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NCBI and Uniprot Product Information
NCBI Description
PhoPQ has been primarily characterized in Salmonella, with significant differences from Escherichia coli. [More information is available at EcoGene: EG10732]. The subunit structure in vivo is not known. [More information is available at EcoCyc: EG10732].
Uniprot Description
Function: Member of the two-component regulatory system PhoQ/PhoP involved in adaptation to low Mg2+ environments and the control of acid resistance genes. In presence of low periplasmic Mg2+ concentrations, PhoQ functions as a membrane-associated protein kinase that undergoes autophosphorylation and subsequently transfers the phosphate to PhoP, which results in the expression of PhoP-activated genes (PAG) and repression of PhoP-repressed genes (PRG). In presence of high periplasmic Mg2+ concentrations, acts as a protein phosphatase that dephosphorylates phospho-PhoP, which results in the repression of PhoP-activated genes and may lead to expression of some PhoP-repressed genes
By similarity. Mediates magnesium influx to the cytosol by activation of mgtA. Promotes expression of the two-component regulatory system rstA/rstB and transcription of the hemL, mgrB, nagA, slyB, vboR and yrbL genes. Ref.7
Catalytic activity: ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Enzyme regulation: Acetyl-CoA acts as a non-competitive inhibitor of the PhoQ autokinase activity. Ref.9
Subunit structure: Homodimer
Probable. Ref.18
Subcellular location: Cell inner membrane; Multi-pass membrane protein Ref.15.
Induction: PhoQ/phoP operon is positively autoregulated by both PhoP and PhoQ in a Mg2+-dependent manner. Ref.7 Ref.9 Ref.10 Ref.14
Miscellaneous: There is a close linkage between the PhoQ/PhoP and Rcs signaling systems, and both signaling systems respond to certain external divalent cations (zinc and magnesium).Two-component regulatory system EvgA/EvgS interacts with PhoQ/PhoP via signal transduction mediated by phospho-EvgA.
Sequence similarities: Contains 1 HAMP domain.Contains 1 histidine kinase domain.
Biophysicochemical propertiesKinetic parameters:KM=20.93 µM for ATP (at 22 degrees Celsius and pH 8, in the presence of 50 mM KCl and 1 mM MgCl2) Ref.9 Ref.17KM=55 µM for ATP (at 22 degrees Celsius and pH 8, in the presence of 25 mM KCl and 0.4 mM MgCl2)
Research Articles on PHOQ
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Product Notes
The PHOQ phoq (Catalog #AAA7153852) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Escherichia coli (strain K12) PHOQ Polyclonal Antibody reacts with E Coli (strain K12) and may cross-react with other species as described in the data sheet. AAA Biotech's PHOQ can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), WB (Western Blot) (ensure identification of antigen). Researchers should empirically determine the suitability of the PHOQ phoq for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHOQ, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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