Rabbit anti-Human PHKG2 Polyclonal Antibody | anti-PHKG2 antibody

PHKG2 (Phosphorylase Kinase Subunit gamma 2, Phosphorylase B Kinase gamma Catalytic Chain Testis/liver Isoform, PHK-gamma-T, GSD9C, PSK-C3) (PE)

Cat. Num. AAA6389338

• Gene Names: PHKG2; GSD9C
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PHKG2; Polyclonal Antibody; PHKG2 (Phosphorylase Kinase Subunit gamma 2; Phosphorylase B Kinase gamma Catalytic Chain Testis/liver Isoform; PHK-gamma-T; GSD9C; PSK-C3) (PE); anti-PHKG2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PHKG2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PHKG2 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human PHKG2, aa1-406 (NP_000285.1).
• Immunogen Sequence: MTLDVGPEDELPDWAAAKEFYQKYDPKDVIGRGVSSVVRRCVHRATGHEFAVKIMEVTAERLSPEQLEEVREATRRETHILRQVAGHPHIITLIDSYESSSFMFLVFDLMRKGELFDYLTEKVALSEKETRSIMRSLLEAVSFLHANNIVHRDLKPENILLDDNMQIRLSDFGFSCHLEPGEKLRELCGTPGYLAPEILKCSMDETHPGYGKEVDLWACGVILFTLLAGSPPFWHRRQILMLRMIMEGQYQFSSPEWDDRSSTVKDLISRLLQVDPEARLTAEQALQHPFFERCEGSQPWNLTPRQRFRVAVWTVLAAGRVALSTHRVRPLTKNALLRDPYALRSVRHLIDNCAFRLYGHWVKKGEQQNRAALFQHRPPGPFPIMGPEEEGDSAAITEDEAVLVLG
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of PHKG2 expression in transfected 293T cell line by PHKG2 polyclonal antibody. Lane 1: PHKG2 transfected lysate (46.4kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-PHKG2 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway The calcium/calmodulin-dependent kinase (CAMK) group consists of 75 kinases regulated by Ca2+/CaM and close relative family (CAMK, CAMKL, DAPK, MAPKAPK).

Product Categories/Family for anti-PHKG2 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 4505785
• NCBI GeneID: 5261
• NCBI Accession #: NP_000285.1
• NCBI GenBank Nucleotide #: NM_000294.1
• UniProt Accession #: P15735; P11800; A8K0C7; B4DEB7; E9PEU3
• Molecular Weight: 43,158 Da
• NCBI Official Full Name: phosphorylase b kinase gamma catalytic chain, liver/testis isoform isoform 1
• NCBI Official Synonym Full Names: phosphorylase kinase catalytic subunit gamma 2
• NCBI Official Symbol: PHKG2
• NCBI Official Synonym Symbols: GSD9C
• NCBI Protein Information: phosphorylase b kinase gamma catalytic chain, liver/testis isoform
• UniProt Protein Name: Phosphorylase b kinase gamma catalytic chain, liver/testis isoform
• Protein Family: Phosphorylase b kinase
• UniProt Gene Name: PHKG2
• UniProt Synonym Gene Names: PHK-gamma-LT; PHK-gamma-T
• UniProt Entry Name: PHKG2_HUMAN

NCBI Description

Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9C, also known as autosomal liver glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]