Western Blot (WB)
(PHF6 antibody (pAb) tested by Western blot. K562 nuclear extract (20 ug) was probed with PHF6 antibody at 1:1000 dilution.)
Rabbit anti-Human PHF6 Polyclonal Antibody | anti-PHF6 antibody
PHF6 antibody (pAb)
Gene Names
PHF6; BFLS; BORJ; CENP-31
Reactivity
Human
Applications
Western Blot
Purity
Affinity Purified
Synonyms
PHF6; Polyclonal Antibody; PHF6 antibody (pAb); PHD Finger Protein 6; CENP-31; anti-PHF6 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-PHF6 antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:500-1:1,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
WB: 1:500-1:1,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
Immunogen
This antibody was generated using a peptide from the N-terminal region of human PHF6 (PHD finger protein 6 isoform 1).
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(PHF6 antibody (pAb) tested by Western blot. K562 nuclear extract (20 ug) was probed with PHF6 antibody at 1:1000 dilution.)
Western Blot (WB)
(PHF6 antibody (pAb) tested by Western blot. K562 nuclear extract (20 ug) was probed with PHF6 antibody at 1:1000 dilution.)
Related Product Information for anti-PHF6 antibody
Short Description: Our PHF6 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Human samples.
Background: PHF6 is a member of the plant homeodomain finger (PHF) family, which has a potential rode in regulation of chromatin accessibility to lineage-specific transcription factors as part of the nucleosome remodeling and deacetylase (NuRD) epigenetic complex. PHF6 has been reported as a tumor suppressor in association with hematopoietic malignancies including T-cell acute lymphoblastic leukemia (T-ALL) and acute myeloid leukemia (AML). PHF6 may have tissue-specific expression, and is overexpressed in both breast and colorectal cancers. Germline mutations in PHF6 are the cause of the Börjeson-Forssman-Lehmann X-linked intellectual disability syndrome (BFLS).
Background: PHF6 is a member of the plant homeodomain finger (PHF) family, which has a potential rode in regulation of chromatin accessibility to lineage-specific transcription factors as part of the nucleosome remodeling and deacetylase (NuRD) epigenetic complex. PHF6 has been reported as a tumor suppressor in association with hematopoietic malignancies including T-cell acute lymphoblastic leukemia (T-ALL) and acute myeloid leukemia (AML). PHF6 may have tissue-specific expression, and is overexpressed in both breast and colorectal cancers. Germline mutations in PHF6 are the cause of the Börjeson-Forssman-Lehmann X-linked intellectual disability syndrome (BFLS).
Product Categories/Family for anti-PHF6 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
41kDa
NCBI Official Full Name
PHD finger protein 6 isoform 1
NCBI Official Synonym Full Names
PHD finger protein 6
NCBI Official Symbol
PHF6
NCBI Official Synonym Symbols
BFLS; BORJ; CENP-31
NCBI Protein Information
PHD finger protein 6
UniProt Protein Name
PHD finger protein 6
Protein Family
UniProt Gene Name
PHF6
UniProt Synonym Gene Names
CENP-31; KIAA1823
UniProt Entry Name
PHF6_HUMAN
NCBI Description
This gene is a member of the plant homeodomain (PHD)-like finger (PHF) family. It encodes a protein with two PHD-type zinc finger domains, indicating a potential role in transcriptional regulation, that localizes to the nucleolus. Mutations affecting the coding region of this gene or the splicing of the transcript have been associated with Borjeson-Forssman-Lehmann syndrome (BFLS), a disorder characterized by cognitive disability, epilepsy, hypogonadism, hypometabolism, obesity, swelling of subcutaneous tissue of the face, narrow palpebral fissures, and large ears. Alternate splicing results in multiple transcript variants, encoding different isoforms. [provided by RefSeq, Jun 2010]
Research Articles on PHF6
Similar Products
Product Notes
The PHF6 phf6 (Catalog #AAA389053) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PHF6 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PHF6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:500-1:1,000 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. Researchers should empirically determine the suitability of the PHF6 phf6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHF6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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