Rabbit anti-Human PHF5A Polyclonal Antibody | anti-PHF5A antibody

PHF5A, CT (PHF5A, PHD finger-like domain-containing protein 5A, Splicing factor 3B-associated 14kD protein) (AP)

Cat. Num. AAA6333365

• Gene Names: PHF5A; INI; Rds3; SF3B7; SAP14b; SF3b14b; bK223H9.2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PHF5A; Polyclonal Antibody; CT (PHF5A; PHD finger-like domain-containing protein 5A; Splicing factor 3B-associated 14kD protein) (AP); Splicing factor 3B-associated 14kD protein; anti-PHF5A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-PHF5A antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PHF5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-110 amino acids from the C-terminal region of human PHF5A.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PHF5A Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PHF5A antibody detected the PHF5A protein (arrow).)

Flow Cytometry (FC/FACS)

(PHF5A Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PHF5A antibody

This gene encodes a subunit of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. The protein encoded by this gene contains a PHD-finger-like domain that is flanked by highly basic N-and C-termini. This protein belongs to the PHD-finger superfamily and may act as a chromatin-associated protein. This gene has several pseudogenes on different chromosomes.

Product Categories/Family for anti-PHF5A antibody

Antibodies; Splicing Factors

References

Kuwasako, K., et al. Proteins 71(4):1617-1636(2008). Will, C.L., et al. RNA 10(6):929-941(2004). Collins, J.E., et al. Genome Biol. 5 (10), R84 (2004): Will, C.L., et al. EMBO J. 21(18):4978-4988(2002). Zhou, Z., et al. Nature 419(6903):182-185(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 14249398
• NCBI GeneID: 84844
• NCBI Accession #: NP_116147.1
• NCBI GenBank Nucleotide #: NM_032758.3
• UniProt Accession #: Q7RTV0; Q9UH06
• Molecular Weight: 12,405 Da
• NCBI Official Full Name: PHD finger-like domain-containing protein 5A
• NCBI Official Synonym Full Names: PHD finger protein 5A
• NCBI Official Symbol: PHF5A
• NCBI Official Synonym Symbols: INI; Rds3; SF3B7; SAP14b; SF3b14b; bK223H9.2
• NCBI Protein Information: PHD finger-like domain-containing protein 5A; PHD finger-like domain protein 5A; PHD-finger 5a; splicing factor 3B associated 14 kDa protein; splicing factor 3B-associated 14 kDa protein; splicing factor 3b, subunit 7
• UniProt Protein Name: PHD finger-like domain-containing protein 5A
• UniProt Gene Name: PHF5A
• UniProt Synonym Gene Names: PHD finger-like domain protein 5A; SF3b14b
• UniProt Entry Name: PHF5A_HUMAN

NCBI Description

This gene encodes a subunit of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. The protein encoded by this gene contains a PHD-finger-like domain that is flanked by highly basic N- and C-termini. This protein belongs to the PHD-finger superfamily and may act as a chromatin-associated protein. This gene has several pseudogenes on different chromosomes. [provided by RefSeq, Jul 2008]

Uniprot Description

PHF5A: Acts as a transcriptional regulator by binding to the GJA1/Cx43 promoter and enhancing its up-regulation by ESR1/ER- alpha. Also involved in pre-mRNA splicing. Belongs to the PHF5 family.

Protein type: RNA-binding; RNA splicing

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: nucleoplasm; nuclear matrix; nuclear speck; snRNP U2; U12-dependent spliceosome

Molecular Function: DNA binding; transcription factor activity

Biological Process: nuclear mRNA splicing, via spliceosome; transcription, DNA-dependent; RNA splicing; positive regulation of transcription, DNA-dependent; gene expression