Rabbit PHB Polyclonal Antibody | anti-PHB antibody

PHB(Phospho-Tyr259) Antibody

Cat. Num. AAA9412237

• Gene Names: PHB; PHB1; HEL-215; HEL-S-54e
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: PHB; Polyclonal Antibody; PHB(Phospho-Tyr259) Antibody; Prohibitin; PHB1; anti-PHB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of PHB only when phosphorylated at tyrosine 259.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 272

• Applicable Applications for anti-PHB antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500~1:1000
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of tyrosine 259 (I-T-Y(p)-L-P) derived from Human PHB.
• Modification: Phospho-Tyr259
• Target Name: PHB
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from MCF, PC12, 293 and 3T3 cells using PHB (Phospho-Tyr259) Antibody.)

Related Product Information for anti-PHB antibody

Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging. 

Product Categories/Family for anti-PHB antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 527498279
• NCBI GeneID: 5245
• NCBI Accession #: NP_001268425.1
• NCBI GenBank Nucleotide #: NM_001281496.1
• UniProt Accession #: P35232; Q4VBQ0; B4DY47
• Molecular Weight: 17,018 Da
• NCBI Official Full Name: prohibitin isoform 1
• NCBI Official Synonym Full Names: prohibitin
• NCBI Official Symbol: PHB
• NCBI Official Synonym Symbols: PHB1; HEL-215; HEL-S-54e
• NCBI Protein Information: prohibitin
• UniProt Protein Name: Prohibitin
• Protein Family: Prohibitin
• UniProt Gene Name: PHB
• UniProt Entry Name: PHB_HUMAN

NCBI Description

This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

PHB: a pleiotropic membrane protein that suppresses cell proliferation, apoptosis and senescence. It plays a role both in maintaining mitochondrial integrity and in cell cycle regulation. Expression increases approximately 3-fold upon entry into G1 phase compared with other phases of the cell cycle. It helps prevent ROS-induced senescence and its expression decreases heterogeneously during cellular aging. Helps maintain the angiogenic capacity of endothelial cells. Up-regulated during activation of primary human T cells via CD3/CD28 pathways. Present in multiple cellular compartments. Large assemblies of PHB and PHB2 localize in the inner membrane of mitochondria. Acts as a mitochondrial chaperone protein, regulates mitochondrial morphogenesis, and helps regulate the organization of mitochondrial DNA. Reported to target lipid rafts. In the nucleus, it has been reported to interact with various transcription factors, modulating their activity. A potential tumor suppressor that functions as a potent transcriptional corepressor for estrogen receptor alpha. It is downregulated by androgens, and represses androgen receptor activity. Co-localizes with Rb in the nucleus and recruits N-CoR and HDAC1 for transcriptional repression. In vivo promoter occupancy studies have suggested that the PHB gene is a direct target of c-Myc. May bind to and enhance the transcriptional activity of p53. Both Phb and Phb2 are present in the circulation and can be internalized by cultured cells. Overexpressed in endometrioid ovarian adenocarcinoma and papillary serous ovarian carcinoma. Mutated in sporadic breast cancer.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor; Cell cycle regulation; Oncoprotein; Chaperone; Motility/polarity/chemotaxis; Apoptosis; Mitochondrial

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: nucleoplasm; mitochondrion; membrane; integral to plasma membrane; mitochondrial inner membrane; cytoplasm; nucleus

Molecular Function: protein binding; enzyme binding; histone deacetylase binding

Biological Process: transcription from RNA polymerase II promoter; osteoblast differentiation; regulation of apoptosis; negative regulation of cell proliferation; progesterone receptor signaling pathway; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; histone deacetylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; signal transduction; negative regulation of transcription, DNA-dependent

Disease: Breast Cancer