Rabbit anti-Human PGR Polyclonal Antibody | anti-PGR antibody

PGR, Phosphorylated (S190) (Progesterone Receptor, NR3C3, PR) (HRP)

Cat. Num. AAA6431373

• Gene Names: PGR; PR; NR3C3
• Reactivity: Human
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: PGR; Polyclonal Antibody; Phosphorylated (S190) (Progesterone Receptor; NR3C3; PR) (HRP); anti-PGR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PGR, phosphorylated S190.
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-PGR antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: 1:100-1:200; WB: 1:500-1:2000; IHC: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Phospho specific peptide corresponding to residues surrounding S190 of human PGR.
• Conjugate: HRP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts from SKOV3 cells using Phospho-PGR-S190 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-PGR-S190 antibody.)

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed MCF cells using Phospho-PGR-S190 antibody.)

Product Categories/Family for anti-PGR antibody

Antibodies; Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 110611914
• NCBI GeneID: 5241
• NCBI Accession #: NP_000917.3
• NCBI GenBank Nucleotide #: NM_000926.4
• UniProt Accession #: P06401; Q9UPF7; A7X8B0
• Molecular Weight: 98,981 Da
• NCBI Official Full Name: progesterone receptor isoform B
• NCBI Official Synonym Full Names: progesterone receptor
• NCBI Official Symbol: PGR
• NCBI Official Synonym Symbols: PR; NR3C3
• NCBI Protein Information: progesterone receptor; nuclear receptor subfamily 3 group C member 3
• UniProt Protein Name: Progesterone receptor
• Protein Family: Protein
• UniProt Gene Name: PGR
• UniProt Synonym Gene Names: NR3C3; PR
• UniProt Entry Name: PRGR_HUMAN

NCBI Description

This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce two isoforms, A and B. The two isoforms are identical except for the additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap. [provided by RefSeq, Jan 2011]

Uniprot Description

PR: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 11q22-q23

Cellular Component: nucleoplasm; mitochondrial outer membrane

Molecular Function: protein binding; ligand-dependent nuclear receptor activity; enzyme binding; DNA binding; zinc ion binding; steroid hormone receptor activity; steroid binding; receptor binding

Biological Process: transcription initiation from RNA polymerase II promoter; progesterone receptor signaling pathway; cell-cell signaling; epithelial cell maturation; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; gene expression; signal transduction; ovulation from ovarian follicle; regulation of epithelial cell proliferation

Disease: Progesterone Resistance