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Western Blot (WB) (Western blot analysis of PGLS Antibody (C-term) in Ramos cell line lysates (35ug/lane). PGLS (arrow) was detected using the purified Pab.)

Rabbit anti-Human PGLS Polyclonal Antibody | anti-PGLS antibody

PGLS, CT (6-phosphogluconolactonase, 6PGL) (MaxLight 490)

Gene Names
PGLS; 6PGL; HEL-S-304
Reactivity
Human
Applications
Western Blot
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
PGLS; Polyclonal Antibody; CT (6-phosphogluconolactonase; 6PGL) (MaxLight 490); anti-PGLS antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PGLS.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Applicable Applications for anti-PGLS antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:50-1:100
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human PGLS (KLH).
Conjugate
MaxLight490
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PGLS Antibody (C-term) in Ramos cell line lysates (35ug/lane). PGLS (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of PGLS Antibody (C-term) in Ramos cell line lysates (35ug/lane). PGLS (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with PGLS Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human brain tissue reacted with PGLS Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PGLS Antibody (C-term) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (PGLS Antibody (C-term) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-PGLS antibody
References
1. Miclet, E., et.al., J. Biol. Chem. 276(37), 34840-34846 (2001).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29.7 kDa (278aa) confirmed by MALDI-TOF
NCBI Official Full Name
6-phosphogluconolactonase
NCBI Official Synonym Full Names
6-phosphogluconolactonase
NCBI Official Symbol
PGLS
NCBI Official Synonym Symbols
6PGL; HEL-S-304
NCBI Protein Information
6-phosphogluconolactonase
UniProt Protein Name
6-phosphogluconolactonase
UniProt Gene Name
PGLS
UniProt Synonym Gene Names
6PGL
UniProt Entry Name
6PGL_HUMAN

Uniprot Description

PGLS: Hydrolysis of 6-phosphogluconolactone to 6- phosphogluconate. Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.

Protein type: Carbohydrate Metabolism - pentose phosphate pathway; Hydrolase; EC 3.1.1.31

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: cytosol

Molecular Function: monosaccharide binding; 6-phosphogluconolactonase activity

Biological Process: pentose-phosphate shunt; carbohydrate metabolic process; pentose-phosphate shunt, oxidative branch; pathogenesis

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Product Notes

The PGLS pgls (Catalog #AAA6492675) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PGLS, CT (6-phosphogluconolactonase, 6PGL) (MaxLight 490) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PGLS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:50-1:100 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PGLS pgls for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PGLS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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