Rabbit anti-Human, Mouse PGK1 Polyclonal Antibody | anti-PGK1 antibody

PGK1, ID (PGK1, PGKA, Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primer recognition protein 2)

Cat. Num. AAA646750

• Gene Names: PGK1; PGKA; MIG10
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: PGK1; Polyclonal Antibody; ID (PGK1; PGKA; Phosphoglycerate kinase 1; Cell migration-inducing gene 10 protein; Primer recognition protein 2); Anti -PGK1; anti-PGK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-PGK1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow cytometry; Immunofluorescence: 1:10-50; Flow Cytometry: 1:10-50
• Immunogen: PGK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124~153 amino acids from the center region of human PGK1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of hPGK1-G132 in Hela(lane 1), HepG2(lane 2) cell line lysates (35ug/lane). PGK1 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of PGK1 (arrow) using rabbit polyclonal PGK1 Antibody (G132) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PGK1 gene.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PGK1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of PGK1 Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of PGK1 Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-PGK1 antibody

Also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. New blood vessel formation or angiogenesis is critical for tumor expansion and metastasis. Lay et al. (2000) showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. They concluded that phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and participates in the angiogenic process as a disulfide reductase.

Product Categories/Family for anti-PGK1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 4505763
• NCBI GeneID: 5230
• NCBI Accession #: NP_000282.1
• NCBI GenBank Nucleotide #: NM_000291.3
• UniProt Accession #: P00558; Q5J7W1; Q6IBT6; Q8NI87; A8K4W6
• Molecular Weight: 44,615 Da
• NCBI Official Full Name: phosphoglycerate kinase 1
• NCBI Official Synonym Full Names: phosphoglycerate kinase 1
• NCBI Official Symbol: PGK1
• NCBI Official Synonym Symbols: PGKA; MIG10
• NCBI Protein Information: phosphoglycerate kinase 1; PRP 2; primer recognition protein 2; cell migration-inducing gene 10 protein
• UniProt Protein Name: Phosphoglycerate kinase 1
• Protein Family: Phosphoglycerate kinase
• UniProt Gene Name: PGK1
• UniProt Synonym Gene Names: PGKA; PRP 2
• UniProt Entry Name: PGK1_HUMAN

NCBI Description

The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq, Oct 2008]

Uniprot Description

Function: In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).

Catalytic activity: ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.

Subunit structure: Monomer.

Subcellular location: Cytoplasm.

Involvement in disease: Phosphoglycerate kinase 1 deficiency (PGK1D) [MIM:300653]: A condition with a highly variable clinical phenotype that includes hemolytic anemia, rhabdomyolysis, myopathy and neurologic involvement. Patients can express one or more of these manifestations.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.25 Ref.26 Ref.27 Ref.28 Ref.29 Ref.30 Ref.33 Ref.34 Ref.35

Sequence similarities: Belongs to the phosphoglycerate kinase family.