Rabbit anti-Human PGBD3 Polyclonal Antibody | anti-PGBD3 antibody

PGBD3, NT (PGBD3, PiggyBac transposable element-derived protein 3) (PE)

Cat. Num. AAA6333012

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PGBD3; Polyclonal Antibody; NT (PGBD3; PiggyBac transposable element-derived protein 3) (PE); PiggyBac transposable element-derived protein 3; anti-PGBD3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PGBD3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PGBD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-49 amino acids from the N-terminal region of human PGBD3.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PGBD3 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the PGBD3 antibody detected the PGBD3 protein (arrow).)

Related Product Information for anti-PGBD3 antibody

The piggyBac family of proteins, found in diverse animals, are transposases related to the transposase of the canonical piggyBac transposon from the moth, Trichoplusia ni. This family also includes genes in several genomes, including human, that appear to have been derived from the piggyBac transposons. This gene belongs to the subfamily of piggyBac transposable element derived (PGBD) genes. The PGBD proteins appear to be novel, with no obvious relationship to other transposases, or other known protein families. This gene overlaps with the ERCC6 gene on chromosome 10, and pseudogenes of this locus have been found on chromosomes 4, 5 and 12.

Product Categories/Family for anti-PGBD3 antibody

Antibodies; Proteins

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Newman, J.C., et al. PLoS Genet. 4 (3), E1000031 (2008): Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006). Sarkar, A., et al. Mol. Genet. Genomics 270(2):173-180(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 284055225
• NCBI GeneID: 267004
• NCBI Accession #: NP_736609.2
• NCBI GenBank Nucleotide #: NM_170753.3
• UniProt Accession #: Q8N328; Q5W0M0; Q6PIH0; B3KQC4
• Molecular Weight: 119,487 Da
• NCBI Official Full Name: piggyBac transposable element-derived protein 3
• NCBI Official Synonym Full Names: piggyBac transposable element derived 3
• NCBI Official Symbol: PGBD3
• NCBI Protein Information: piggyBac transposable element-derived protein 3
• UniProt Protein Name: PiggyBac transposable element-derived protein 3
• Protein Family: PiggyBac transposable element-derived protein
• UniProt Gene Name: PGBD3

NCBI Description

This gene is a member of a small family of genes derived from piggyBac transposable elements. The encoded protein contains a zinc-ribbon domain characteristic of transposon-derived proteins and may function as a regulator of transcription. Alternative splicing occurs between a splice site from exon 5 of the adjacent upstream gene 'excision repair cross-complementation group 6' (ERCC6, GeneID: 2074) and the 3' splice site upstream of the open reading frame (ORF) of this gene, which activates the alternative polyadenylation site downstream of the piggyback-derived-3 ORF. The resulting transcripts encode a fusion protein that shares sequence with the product of each individual gene. Pseudogenes for this gene are defined on chromosomes 4, 5 and 12. [provided by RefSeq, Mar 2016]

Uniprot Description

PGBD3:

Chromosomal Location of Human Ortholog: 10q11.23

Cellular Component: nucleus

Molecular Function: DNA binding