Rabbit anti-Human PGAP3 Polyclonal Antibody | anti-PGAP3 antibody

PGAP3, ID (PGAP3, CAB2, PERLD1, Post-GPI attachment to proteins factor 3, COS16 homolog, Gene coamplified with ERBB2 protein, PER1-like domain-containing protein 1) (Azide free) (HRP)

Cat. Num. AAA6332984

• Gene Names: PGAP3; CAB2; PERLD1; PP1498; hCOS16; AGLA546
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PGAP3; Polyclonal Antibody; ID (PGAP3; CAB2; PERLD1; Post-GPI attachment to proteins factor 3; COS16 homolog; Gene coamplified with ERBB2 protein; PER1-like domain-containing protein 1) (Azide free) (HRP); PER1-like domain-containing protein 1; anti-PGAP3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PGAP3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PGAP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-169 amino acids from the Central region of human PGAP3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PGAP3 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PGAP3 antibody detected the PGAP3 protein (arrow).)

Immunohistochemistry (IHC)

(PGAP3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PGAP3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PGAP3 antibody

PGAP3 is involved in the lipid remodeling steps of GPI-anchor maturation. Lipid remodeling steps consist in the generation of 2 saturated fatty chains at the sn-2 position of GPI-anchors proteins. Required for phospholipase A2 activity that removes an acyl-chain at the sn-2 position of GPI-anchors during the remodeling of GPI (Probable).

Product Categories/Family for anti-PGAP3 antibody

Antibodies; Proteins

References

Bailey, S.D., et al. Diabetes Care (2010). In press: Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Mavaddat, N., et al. Cancer Epidemiol. Biomarkers Prev. 18(1):255-259(2009). Maeda, Y., et al. Mol. Biol. Cell 18(4):1497-1506(2007). Benusiglio, P.R., et al. Br. J. Cancer 95(12):1689-1695(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 45505180
• NCBI GeneID: 93210
• NCBI Accession #: NP_219487.3
• NCBI GenBank Nucleotide #: NM_033419.4
• UniProt Accession #: Q96FM1; Q86Z03; Q8NBJ8; B4DGK7
• Molecular Weight: 36475
• NCBI Official Full Name: post-GPI attachment to proteins factor 3 isoform 1
• NCBI Official Synonym Full Names: post-GPI attachment to proteins 3
• NCBI Official Symbol: PGAP3
• NCBI Official Synonym Symbols: CAB2; PERLD1; PP1498; hCOS16; AGLA546
• NCBI Protein Information: post-GPI attachment to proteins factor 3
• UniProt Protein Name: Post-GPI attachment to proteins factor 3
• Protein Family: Post-GPI attachment to proteins factor
• UniProt Gene Name: PGAP3
• UniProt Synonym Gene Names: CAB2; PERLD1; hCOS16

NCBI Description

This gene encodes a glycosylphosphatidylinositol (GPI)-specific phospholipase that primarily localizes to the Golgi apparatus. This ubiquitously expressed gene is predicted to encode a seven-transmembrane protein that removes unsaturated fatty acids from the sn-2 position of GPI. The remodeling of the constituent fatty acids on GPI is thought to be important for the proper association between GPI-anchored proteins and lipid rafts. The tethering of proteins to plasma membranes via posttranslational GPI-anchoring is thought to play a role in protein sorting and trafficking. Mutations in this gene cause the autosomal recessive neurologic disorder hyperphosphatasia with mental retardation syndrome 4 (HPMRS4). Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Apr 2014]

Uniprot Description

PGAP3: Involved in the lipid remodeling steps of GPI-anchor maturation. Lipid remodeling steps consist in the generation of 2 saturated fatty chains at the sn-2 position of GPI-anchors proteins. Required for phospholipase A2 activity that removes an acyl-chain at the sn-2 position of GPI-anchors during the remodeling of GPI (Probable). Belongs to the PGAP3 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: Golgi membrane; integral component of membrane; intrinsic component of endoplasmic reticulum membrane

Molecular Function: hydrolase activity, acting on ester bonds

Biological Process: GPI anchor biosynthetic process; GPI anchor metabolic process

Disease: Hyperphosphatasia With Mental Retardation Syndrome 4