Rabbit anti-Human PFKP Polyclonal Antibody | anti-PFKP antibody

PFKP, CT (PFKP, PFKF, 6-phosphofructokinase type C, 6-phosphofructokinase, platelet type, Phosphofructo-1-kinase isozyme C, Phosphofructokinase 1, Phosphohexokinase) (PE)

Cat. Num. AAA6332902

• Gene Names: PFKP; PFKF; PFK-C
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: PFKP; Polyclonal Antibody; CT (PFKP; PFKF; 6-phosphofructokinase type C; 6-phosphofructokinase; platelet type; Phosphofructo-1-kinase isozyme C; Phosphofructokinase 1; Phosphohexokinase) (PE); Phosphohexokinase; anti-PFKP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PFKP.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PFKP antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 754-784 in the C-terminal region of human PFKP, UniProt Accession #Q01813.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in T47D cell line lysates (35ug/lane) usingMBS6008356. This demonstrates that MBS6008356 detected the PFKP protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human cancer tissue reacted withMBS6008356, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of MBS6008356 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-PFKP antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).

Product Categories/Family for anti-PFKP antibody

Antibodies; Protein Kinases

References

1. Adam, G.C., et al., Mol. Cell Proteomics 1(10):828-835 (2002). 2. Bonaldo, M.F., et al., Genome Res. 6(9):791-806 (1996). 3. Morrison, N., et al., Hum. Genet. 89(1):105-106 (1992). 4. Simpson, C.J., et al., Biochem. Biophys. Res. Commun. 180(1):197-203 (1991). 5. Nakajima, H., et al., FEBS Lett. 223(1):113-116 (1987).

NCBI and Uniprot Product Information

• NCBI GI #: 334191701
• NCBI GeneID: 5214
• NCBI Accession #: NP_001229268.1
• NCBI GenBank Nucleotide #: NM_001242339.1
• UniProt Accession #: Q01813; Q5VSR7; Q5VSR8; B3KS15
• Molecular Weight: 85,596 Da
• NCBI Official Full Name: 6-phosphofructokinase type C isoform 2
• NCBI Official Synonym Full Names: phosphofructokinase, platelet
• NCBI Official Symbol: PFKP
• NCBI Official Synonym Symbols: PFKF; PFK-C
• NCBI Protein Information: 6-phosphofructokinase type C; phosphohexokinase; phosphofructokinase 1; phosphofructo-1-kinase isozyme C; Phosphofructokinase, platelet type; 6-phosphofructokinase, platelet type
• UniProt Protein Name: 6-phosphofructokinase type C
• Protein Family: ATP-dependent 6-phosphofructokinase
• UniProt Gene Name: PFKP
• UniProt Synonym Gene Names: PFKF; PFK-C
• UniProt Entry Name: K6PP_HUMAN

NCBI Description

The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM; MIM 610681) and liver (PFKL; MIM 171860) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora (1981) [PubMed 6451249] determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.[supplied by OMIM, Mar 2008]

Uniprot Description

PFKP: phosphofructokinase, platelet type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts.

Protein type: Carbohydrate Metabolism - pentose phosphate pathway; Kinase, other; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - fructose and mannose; EC 2.7.1.11; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 10p15.3-p15.2

Cellular Component: membrane; nucleus; cytosol

Molecular Function: metal ion binding; protein complex binding; 6-phosphofructokinase activity; ATP binding

Biological Process: glycolysis; carbohydrate metabolic process; carbohydrate phosphorylation; glucose metabolic process; pathogenesis; fructose 6-phosphate metabolic process